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Product Info Summary
| SKU: | M00269-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MiTF Rabbit Monoclonal Antibody
SKU/Catalog Number
M00269-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MiTF Rabbit Monoclonal Antibody catalog # M00269-2. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MiTF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00269-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOED-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MiTF
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00269-2 is reactive to MITF in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
58795 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00269-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of MiTF expression in A375 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MITF (Source: Uniprot.org, NCBI)
Gene Name
MITF
Full Name
Microphthalmia-associated transcription factor
Weight
58795 MW
Superfamily
MiT/TFE family
Alternative Names
Microphthalmia-associated transcription factor;Class E basic helix-loop-helix protein 32;bHLHe32;MITF;BHLHE32; MITF CMM8, COMMAD, MI, WS2, WS2A, bHLHe32 melanocyte inducing transcription factor microphthalmia-associated transcription factor|class E basic helix-loop-helix protein 32|melanogenesis associated transcription factor|microphtalmia-associated transcription factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MITF, check out the MITF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MITF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MiTF Rabbit Monoclonal Antibody (M00269-2)
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1 Customer Q&As for Anti-MiTF Rabbit Monoclonal Antibody
Question
We are currently using anti-MiTF Rabbit Monoclonal antibody M00269-2 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The anti-MiTF Rabbit Monoclonal antibody (M00269-2) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-24
