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MITF Antibodies
AND MITF ELISA kits, MITF Proteins
Many biological assays use antibodies to detect MITF, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with MITF in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop MITF antibodies...
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MITF Infographic by Boster Bio
All MITF Products
Product
Figures
Specificity
Applications
Price
Dog, Human
IHC
$486
Cat # M00269-1
100ug/vial
Cat # M00269-1
100ug/vial
Human, Mouse
ELISA
$480
Cat # EKC1370
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1370
1 kit, containing one 96-well plate and all necessary reagents
Human, Mouse
ELISA
$540
Cat # EKC2088
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2088
1 kit, containing two 96-well plates and all necessary reagents
MITF Overview
Facts about Microphthalmia-associated transcription factor.
Microphthalmia-associated transcription factor (MITF)
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR).
Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a crucial role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Gene Information
| Human | |
|---|---|
| Gene Name: | MITF |
| Uniprot: | O75030 |
| Entrez: | 4286 |
Family
| Belongs to: |
|---|
| MiT/TFE family |
Alternative Names
bHLHe32; bHLHe32MI; Class E basic helix-loop-helix protein 32; MI; microphthalmia-associated transcription factor; MITF; Waardenburg syndrome, type 2A; WS2A
Molecular Weight
Mass (kDA):
58.795 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 3p13 |
| Sequence: | 3; NC_000003.12 (69739464..69968337) |
Tissue Specificity
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.
Subcellular Localizations
Nucleus.
Diseases
- Melanoma
- Microphthalmos
- Neoplasms
- Skin Neoplasms
- Waardenburg Syndrome
- Malignant Neoplasms
- Malignant Paraganglionic Neoplasm
- Melanoma, Experimental
- Neoplasm Metastasis
- Crest Syndrome
- Hypopigmentation Disorder
- Complete Hearing Loss
- B16 Malignant Melanoma
Pathways
- Pigmentation
- Melanocyte Differentiation
- Cell Proliferation
- Cell Cycle
- Localization
- Cell Differentiation
- Osteoclast Differentiation
- Cell Growth
- Eye Development
- Cell Development
- Pathogenesis
- Secretion
- Transdifferentiation
PTMs
- Phosphorylation
- Cleavage
- Sumoylation
- Acetylation
- Methylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MITF
bosterbio.com/bosterbio-gene-info-cards/MITF
References
PMID: 9647758 by Amae S., et al. Identification of a novel isoform of microphthalmia-associated transcription factor that is enriched in retinal pigment epithelium.
PMID: 8069297 by Tachibana M., et al. Cloning of MITF, the human homolog of the mouse microphthalmia gene and assignment to chromosome 3p14.1-p12.3.