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Product Info Summary
| SKU: | M00269 |
|---|---|
| Size: | 100 µg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MITF Antibody (Monoclonal, ABM1H91)
SKU/Catalog Number
M00269
Size
100 µg
Form
Liquid
Description
Boster Bio Anti-MITF Antibody (Monoclonal, ABM1H91) (Catalog# M00269). Tested in IHC, WB application(s). This antibody reacts with Mouse, Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-MITF Antibody (Monoclonal, ABM1H91) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00269)
Host
Mouse
Contents
100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.
Clonality
Monoclonal
Clone Number
ABM1H91
Isotype
IgG1 Kappa
Immunogen
A partial length recombinant MITF protein (amino acids 150-427)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00269 is reactive to MITF in Human, Mouse
Applications
M00269 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
58795 MW
Background of MITF
The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors.HNF-1 (), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 2-4 µg/ml, IHC: 10 µg/ml
Validation Images & Assay Conditions
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2 Immunohistochemical analysis of MITF in human Skin tissue using MITF antibody (Clone: ABM1H91) at 10 &mu
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Western blot analysis of MITF. Anti-MITF antibody (Clone: ABM1H91) was tested at 4 &mu
Protein Target Info & Infographic
Gene/Protein Information For MITF (Source: Uniprot.org, NCBI)
Gene Name
MITF
Full Name
Microphthalmia-associated transcription factor
Weight
58795 MW
Superfamily
MiT/TFE family
Alternative Names
bHLHe32; bHLHe32MI; Class E basic helix-loop-helix protein 32; MI; microphthalmia-associated transcription factor; MITF; Waardenburg syndrome, type 2A; WS2A MITF CMM8, COMMAD, MI, WS2, WS2A, bHLHe32 melanocyte inducing transcription factor microphthalmia-associated transcription factor|class E basic helix-loop-helix protein 32|melanogenesis associated transcription factor|microphtalmia-associated transcription factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MITF, check out the MITF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MITF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MITF Antibody (Monoclonal, ABM1H91) (M00269)
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No publications found for M00269
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-MITF Antibody (Monoclonal, ABM1H91)
Question
I was wanting to use your anti-MITF antibody (Monoclonal, ABM1H91) for WB for mouse thalamus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse thalamus identification?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
It shows on the product datasheet, M00269 anti-MITF antibody (Monoclonal, ABM1H91) has been tested for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse thalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-02
Question
Is a blocking peptide available for product anti-MITF antibody (Monoclonal, ABM1H91) (M00269)?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
We do provide the blocking peptide for product anti-MITF antibody (Monoclonal, ABM1H91) (M00269). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-23
Question
See below the WB image, lot number and protocol we used for thalamus using anti-MITF antibody (Monoclonal, ABM1H91) M00269. Please let me know if you require anything else.
M. Anderson
Verified customer
Asked: 2017-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-23
Question
We want to test anti-MITF antibody (Monoclonal, ABM1H91) M00269 on mouse thalamus for research purposes, then I may be interested in using anti-MITF antibody (Monoclonal, ABM1H91) M00269 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Moore
Verified customer
Asked: 2015-08-06
Answer
The products we sell, including anti-MITF antibody (Monoclonal, ABM1H91) M00269, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-08-06
