Anti-MITF Antibody (Monoclonal, ABM1H91)

Product Name

Anti-MITF Antibody (Monoclonal, ABM1H91)

View all MITF Antibodies

SKU/Catalog Number

M00269

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-MITF Antibody (Monoclonal, ABM1H91) (Catalog# M00269). Tested in IHC, WB application(s). This antibody reacts with Mouse, Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-MITF Antibody (Monoclonal, ABM1H91) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00269)

Host

Mouse

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Monoclonal

Clone Number

ABM1H91

Isotype

IgG1 Kappa

Immunogen

A partial length recombinant MITF protein (amino acids 150-427)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00269 is reactive to MITF in Human, Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

58795 MW

Background of MITF

The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors.HNF-1 (), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00269 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 2-4 µg/ml, IHC: 10 µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For MITF (Source: Uniprot.org, NCBI)

Alternative Names

Microphthalmia-associated transcription factor;Class E basic helix-loop-helix protein 32;bHLHe32;MITF;BHLHE32; MITF CMM8, COMMAD, MI, WS2, WS2A, bHLHe32 melanocyte inducing transcription factor microphthalmia-associated transcription factor|class E basic helix-loop-helix protein 32|melanogenesis associated transcription factor|microphtalmia-associated transcription factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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