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Product Info Summary
| SKU: | A11898-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-METAP1D Antibody Picoband®
View all Methionine Aminopeptidase 1D/MAP1D Antibodies
SKU/Catalog Number
A11898-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-METAP1D Antibody Picoband® catalog # A11898-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-METAP1D Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11898-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human METAP1D recombinant protein (Position: Q36-A335).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11898-1 is reactive to METAP1D in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
37.262kDa
Background of Methionine Aminopeptidase 1D/MAP1D
The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11898-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, human K562 whole cell, human 293T whole cell, human HepG2 whole cell, human THP-1 whole cell, human Hela whole cell, rat liver tissue, rat RH35 whole cell, mouse Neuro-2a whole cell
IHC: human colorectal adenocarcinoma tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of METAP1D using anti-METAP1D antibody (A11898-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: human Hela whole cell lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat RH35 whole cell lysates,
Lane 9: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METAP1D antigen affinity purified polyclonal antibody (Catalog # A11898-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for METAP1D at approximately 37 kDa. The expected band size for METAP1D is at 37 kDa.
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Figure 2. IHC analysis of METAP1D using anti-METAP1D antibody (A11898-1).
METAP1D was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METAP1D Antibody (A11898-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For METAP1D (Source: Uniprot.org, NCBI)
Gene Name
METAP1D
Full Name
Methionine aminopeptidase 1D, mitochondrial
Weight
37.262kDa
Superfamily
peptidase M24A family
Alternative Names
Solute carrier family 2, facilitated glucose transporter member 6; Glucose transporter type 6; GLUT-6; Glucose transporter type 9; GLUT-9; SLC2A6; GLUT9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on METAP1D, check out the METAP1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for METAP1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-METAP1D Antibody Picoband® (A11898-1)
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