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Product Info Summary
| SKU: | M03266-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Mesothelin Monoclonal Antibody
View all Mesothelin Antibodies
SKU/Catalog Number
M03266-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Mesothelin Monoclonal Antibody catalog # M03266-1. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mesothelin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03266-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEIO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Mesothelin Membrane-anchored forms may play a role in cellular adhesion.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03266-1 is reactive to MSLN in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
23147 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03266-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Mesothelin expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MSLN (Source: Uniprot.org, NCBI)
Gene Name
MSLN
Full Name
Mesothelin
Weight
23147 MW
Superfamily
mesothelin family
Alternative Names
Claudin-5;Transmembrane protein deleted in VCFS;TMDVCF;CLDN5;AWAL, TMVCF; MSLN MPF, SMRP mesothelin mesothelin|CAK1 |megakaryocyte potentiating factor|pre-pro-megakaryocyte-potentiating factor|soluble MPF mesothelin related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MSLN, check out the MSLN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MSLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mesothelin Monoclonal Antibody (M03266-1)
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No publications found for M03266-1
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Mesothelin Monoclonal Antibody
Question
My boss were content with the WB result of your anti-Mesothelin Monoclonal antibody. However we have been able to see positive staining in tear cell membrane using this antibody. Is that expected? Could you tell me where is MSLN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
According to literature, tear does express MSLN. Generally MSLN expresses in cell membrane. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2020-04-21
Question
I was wanting to use your anti-Mesothelin Monoclonal antibody for WB for human tear on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tear identification?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
You can see on the product datasheet, M03266-1 anti-Mesothelin Monoclonal antibody has been validated for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tear in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
I am looking for to test anti-Mesothelin Monoclonal antibody M03266-1 on human tear for research purposes, then I may be interested in using anti-Mesothelin Monoclonal antibody M03266-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Lewis
Verified customer
Asked: 2020-01-27
Answer
The products we sell, including anti-Mesothelin Monoclonal antibody M03266-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-27
Question
I see that the anti-Mesothelin Monoclonal antibody M03266-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-28
Question
Would M03266-1 anti-Mesothelin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
As indicated on the product datasheet, M03266-1 anti-Mesothelin Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-14
Question
Is there a BSA free version of anti-Mesothelin Monoclonal antibody M03266-1 available?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Mesothelin Monoclonal antibody M03266-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-03
Question
We have been able to see staining in human carcinoma. Do you have any suggestions? Is anti-Mesothelin Monoclonal antibody supposed to stain carcinoma positively?
A. Yang
Verified customer
Asked: 2018-11-08
Answer
According to literature carcinoma does express MSLN. According to Uniprot.org, MSLN is expressed in right uterine tube, pancreatic cancer, carcinoma, lung spleen, pancreas placenta, pancreatic tumor, tear, among other tissues. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2018-11-08
Question
Is this M03266-1 anti-Mesothelin Monoclonal antibody reactive to the isotypes of MSLN?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
The immunogen of M03266-1 anti-Mesothelin Monoclonal antibody is A synthesized peptide derived from human Mesothelin Membrane-anchored forms may play a role in cellular adhesion. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-03
Question
We are currently using anti-Mesothelin Monoclonal antibody M03266-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
J. Johnson
Verified customer
Asked: 2017-12-14
Answer
The anti-Mesothelin Monoclonal antibody (M03266-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-14
Question
My question regarding product M03266-1, anti-Mesothelin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Huang
Verified customer
Asked: 2017-08-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03266-1 anti-Mesothelin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tear using anti-Mesothelin Monoclonal antibody M03266-1. Let me know if you need anything else.
C. Johnson
Verified customer
Asked: 2016-09-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-09-27
Question
I have attached the WB image, lot number and protocol we used for tear using anti-Mesothelin Monoclonal antibody M03266-1. Please let me know if you require anything else.
B. Roberts
Verified customer
Asked: 2016-06-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-06-16
Question
We bought anti-Mesothelin Monoclonal antibody for IP on tear in the past. I am using human, and We intend to use the antibody for WB next. My question regards examining tear as well as pancreas placenta in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
N. Miller
Verified customer
Asked: 2015-07-16
Answer
I have checked the website and datasheets of our anti-Mesothelin Monoclonal antibody and I see that M03266-1 has been tested on human in both IP and WB. Thus M03266-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-07-16
Question
Will anti-Mesothelin Monoclonal antibody M03266-1 work for WB with tear?
C. Wu
Verified customer
Asked: 2014-07-17
Answer
According to the expression profile of tear, MSLN is highly expressed in tear. So, it is likely that anti-Mesothelin Monoclonal antibody M03266-1 will work for WB with tear.
Boster Scientific Support
Answered: 2014-07-17
Question
Is a blocking peptide available for product anti-Mesothelin Monoclonal antibody (M03266-1)?
M. Moore
Verified customer
Asked: 2013-02-07
Answer
We do provide the blocking peptide for product anti-Mesothelin Monoclonal antibody (M03266-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-02-07
