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Product Info Summary
| SKU: | A02033-3 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Melan-A/MLANA Antibody Picoband®
View all Melan-A/MART-1 Antibodies
SKU/Catalog Number
A02033-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Melan-A/MLANA Antibody Picoband® catalog # A02033-3. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Melan-A/MLANA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02033-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Melan-A/MLANA recombinant protein (Position: M1-L37).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02033-3 is reactive to MLANA in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
13 kDa
Calculated molecular weight
37492 MW
Background of Melan-A/MART-1
MLANA (Melan-A, also known as MART1; MART-1), located on 9p24.1, is a Protein Coding gene. The gene produces a 13157 Da protein composed of 118 amino acids. MLANA is a hydrophobic transmembrane protein without glycosylation sites highly enriched in early melanosomes. MLANA is expressed in skin and retinae melanocytes and the majority of melanoma tumors, but it is absent from other tissues and tumors. Diseases such as Melanoma In Congenital Melanocytic Nevus and Angiomyolipoma are associated with MLANA. The related pathways of MLANA include NF-kappaB Signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02033-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: mouse heart tissue
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Melan-A/MLANA using anti-Melan-A/MLANA antibody (A02033-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Melan-A/MLANA antigen affinity purified polyclonal antibody (Catalog # A02033-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Melan-A/MLANA at approximately 13 kDa. The expected band size for Melan-A/MLANA is at 13 kDa.
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Figure 2. Flow Cytometry analysis of HEL cells using anti-Melan-A/MLANA antibody (A02033-3).
Overlay histogram showing HEL cells stained with A02033-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Melan-A/MLANA Antibody (A02033-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MLANA (Source: Uniprot.org, NCBI)
Gene Name
MLANA
Full Name
Melanoma antigen recognized by T-cells 1
Weight
37492 MW
Alternative Names
Tripartite motif-containing protein 6; RING finger protein 89; RING-type E3 ubiquitin transferase TRIM6; TRIM6; RNF89 MLANA MART-1, MART1 melan-A melanoma recognized by T-cells 1| LB39-AA| SK29-AA|epididymis secretory sperm binding protein|protein Melan-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MLANA, check out the MLANA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MLANA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Melan-A/MLANA Antibody Picoband® (A02033-3)
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