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Product Info Summary
| SKU: | A00894 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-CALR/Calreticulin Antibody
View all Calreticulin Antibodies
SKU/Catalog Number
A00894
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CALR/Calreticulin Antibody (Catalog # A00894). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CALR/Calreticulin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00894)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CALR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00894 is reactive to CALR in Human, Mouse, Rat
Applications
A00894 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
48142 MW
Background of Calreticulin
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis By similarity.
McCauliffe D.P., J. Clin. Invest. 85:1379-1391(1990).
Rokeach L.A., J. Immunol. 147:3031-3039(1991).
McCauliffe D.P., J. Biol. Chem. 267:2557-2562(1992).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using CALR antibody A00894.
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Western blot analysis of extracts from COS-7 cells, using CALR antibody A00894.
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Immunofluorescence analysis of NIH/3T3 cells, using CALR antibody A00894.
Protein Target Info & Infographic
Gene/Protein Information For CALR (Source: Uniprot.org, NCBI)
Gene Name
CALR
Full Name
Calreticulin
Weight
48142 MW
Superfamily
calreticulin family
Alternative Names
Autoantigen Ro; CALR; Calregulin; Calreticulin; cC1qR; CRP55; CRT; CRTC; Endoplasmic reticulum resident protein 60; ERp60; FLJ26680; grp60; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA; SSAHACBP; Vasostatin CALR CRT, HEL-S-99n, RO, SSA, cC1qR calreticulin calreticulin|CRP55|ERp60|HACBP|Sicca syndrome antigen A (autoantigen Ro; calreticulin)|calregulin|endoplasmic reticulum resident protein 60|epididymis secretory sperm binding protein Li 99n|grp60
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CALR, check out the CALR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CALR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CALR/Calreticulin Antibody (A00894)
Hello CJ!
A00894 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu C, Dong S, Li Y. Int J Mol Med. 2015 Apr;35(4):893-900. Doi: 10.3892/Ijmm.2015.2105. Epub 2015 Feb 18. Effects Of Mirna-455 On Cardiac Hypertrophy Induced By Pressure Overload.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CALR/Calreticulin Antibody
Question
We are currently using anti-CALR/Calreticulin antibody A00894 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The anti-CALR/Calreticulin antibody (A00894) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-10
Question
We bought anti-CALR/Calreticulin antibody for IHC on liver a few months ago. I am using mouse, and I plan to use the antibody for WB next. My lab would like examining liver as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
I took a look at the website and datasheets of our anti-CALR/Calreticulin antibody and it appears that A00894 has been tested on mouse in both IHC and WB. Thus A00894 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-16
Question
Does anti-CALR/Calreticulin antibody A00894 work for IHC with colon carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
According to the expression profile of colon carcinoma, CALR is highly expressed in colon carcinoma. So, it is likely that anti-CALR/Calreticulin antibody A00894 will work for IHC with colon carcinoma.
Boster Scientific Support
Answered: 2019-10-23
Question
Will A00894 anti-CALR/Calreticulin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
It shows on the product datasheet, A00894 anti-CALR/Calreticulin antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-11
Question
Please see the WB image, lot number and protocol we used for colon carcinoma using anti-CALR/Calreticulin antibody A00894. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-25
Question
I was wanting to use your anti-CALR/Calreticulin antibody for IHC for rat colon carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat colon carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
As indicated on the product datasheet, A00894 anti-CALR/Calreticulin antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-CALR/Calreticulin antibody A00894. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-06
Question
I see that the anti-CALR/Calreticulin antibody A00894 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-20
Question
My question regarding product A00894, anti-CALR/Calreticulin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Wu
Verified customer
Asked: 2019-01-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00894 anti-CALR/Calreticulin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-17
Question
Is a blocking peptide available for product anti-CALR/Calreticulin antibody (A00894)?
G. Huang
Verified customer
Asked: 2018-09-28
Answer
We do provide the blocking peptide for product anti-CALR/Calreticulin antibody (A00894). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-28
Question
We have seen staining in mouse eye. Do you have any suggestions? Is anti-CALR/Calreticulin antibody supposed to stain eye positively?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
According to literature eye does express CALR. According to Uniprot.org, CALR is expressed in thyroid gland, eye, pancreas skin, placenta, liver, brain, cajal-retzius cell fetal brain cortex, keratinocyte, colon carcinoma, among other tissues. Regarding which tissues have CALR expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 9150948
Eye, Pancreas, and Skin, Pubmed ID: 15489334
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 7841019, 11322874
Boster Scientific Support
Answered: 2017-10-25
Question
I am looking for to test anti-CALR/Calreticulin antibody A00894 on rat colon carcinoma for research purposes, then I may be interested in using anti-CALR/Calreticulin antibody A00894 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
The products we sell, including anti-CALR/Calreticulin antibody A00894, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-28
Question
Is this A00894 anti-CALR/Calreticulin antibody reactive to the isotypes of CALR?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
The immunogen of A00894 anti-CALR/Calreticulin antibody is Synthesized peptide derived from internal of human CALR. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-13
Question
you antibody using your anti-CALR/Calreticulin antibody for antigen presentation: folding studies. Has this antibody been tested with western blotting on human heart tissue? We would like to see some validation images before ordering.
D. Yang
Verified customer
Asked: 2016-10-05
Answer
Thank you for your inquiry. This A00894 anti-CALR/Calreticulin antibody is validated on human heart tissue. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-10-05
Question
Our lab were content with the WB result of your anti-CALR/Calreticulin antibody. However we have seen positive staining in placenta endoplasmic reticulum lumen using this antibody. Is that expected? Could you tell me where is CALR supposed to be expressed?
S. Roberts
Verified customer
Asked: 2015-09-23
Answer
Based on literature, placenta does express CALR. Generally CALR expresses in endoplasmic reticulum lumen. Regarding which tissues have CALR expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 9150948
Eye, Pancreas, and Skin, Pubmed ID: 15489334
Keratinocyte, Pubmed ID: 1286667
Liver, Pubmed ID: 19159218, 24275569
Placenta, Pubmed ID: 7841019, 11322874
Boster Scientific Support
Answered: 2015-09-23
Question
Do you have a BSA free version of anti-CALR/Calreticulin antibody A00894 available?
C. Baker
Verified customer
Asked: 2014-10-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CALR/Calreticulin antibody A00894 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-10-22
