Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody

Product Name

Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody

View all Melan-A/MART-1 Antibodies

SKU/Catalog Number

M02033

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody (Catalog # M02033). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Horse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-MART-1 / Melan-A / MLANA (Melanoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02033)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM342

Isotype

IgG2b, lambda

Immunogen

Recombinant hMART-1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02033 is reactive to MLANA in Human, Horse

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

13157 MW

Background of Melan-A/MART-1

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02033 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For MLANA (Source: Uniprot.org, NCBI)

Alternative Names

Melanoma antigen recognized by T-cells 1;MART-1;Antigen LB39-AA;Antigen SK29-AA;Protein Melan-A;MLANA;MART1; MLANA MART-1, MART1 melan-A melanoma recognized by T-cells 1| LB39-AA| SK29-AA|epididymis secretory sperm binding protein|protein Melan-A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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