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Product Info Summary
| SKU: | A00292 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MEK1/MAP2K1 Antibody Picoband®
SKU/Catalog Number
A00292
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MEK1/MAP2K1 Antibody Picoband® catalog # A00292. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MEK1/MAP2K1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00292)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MEK1/MAP2K1 recombinant protein (Position: M1-V393).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00292 is reactive to MAP2K1 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
43.439kDa
Background of MAP2K1
Dual specificity mitogen-activated protein kinase kinase 1 is an enzyme that in humans is encoded by the MAP2K1 gene. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein(MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon activation by a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Rampoldi et al.(1997) localized the MAP2K1 gene to 15q22.1-q22.33.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00292 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human Daudi whole cell, rat lung tissue, rat brain tissue
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-MEK1/MAP2K1 antibody (A00292).
Overlay histogram showing 293T cells stained with A00292 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEK1/MAP2K1 Antibody (A00292, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MAP2K1 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K1
Full Name
Dual specificity mitogen-activated protein kinase kinase 1
Weight
43.439kDa
Superfamily
protein kinase superfamily
Alternative Names
Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD278; ICOS; AILIM MAP2K1 CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1 mitogen-activated protein kinase kinase 1 dual specificity mitogen-activated protein kinase kinase 1|ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K1, check out the MAP2K1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK1/MAP2K1 Antibody Picoband® (A00292)
Hello CJ!
A00292 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of the PI3K pathway sensitizes fludarabine-induced apoptosis in human leukemic cells through an inactivation of MAPK-dependent pathway
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