Anti-MP2K1 Antibody

MAP2K1 antibody

Boster Bio Anti-MP2K1 Antibody (Catalog # M00292-3). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00292-3
Size: 50 µl
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, WB

Product Name

Anti-MP2K1 Antibody

View all MAP2K1 Antibodies

SKU/Catalog Number

M00292-3

Size

50 µl

Description

Boster Bio Anti-MP2K1 Antibody (Catalog # M00292-3). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-MP2K1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00292-3)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1678CT373.38.18

Isotype

IgG1,k

Immunogen

This MP2K1 antibody is generated from a mouse immunized with a recombinant protein between 1-393 amino acids from human MP2K1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00292-3 is reactive to MAP2K1 in Human, Mouse, Rat

Reconstitution

Calculated molecular weight

43439 Da

Background of MAP2K1

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00292-3 is guaranteed for Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:4000
FC: 1:25

Validation Images & Assay Conditions

Gene/Protein Information For MAP2K1 (Source: Uniprot.org, NCBI)

Gene Name

MAP2K1

Full Name

Dual specificity mitogen-activated protein kinase kinase 1

Weight

43439 Da

Superfamily

protein kinase superfamily

Alternative Names

Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, 2.7.12.2, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1, MEK1, PRKMK1 MAP2K1 CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1 mitogen-activated protein kinase kinase 1 dual specificity mitogen-activated protein kinase kinase 1|ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAP2K1, check out the MAP2K1 Infographic

MAP2K1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAP2K1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00292-3-50ul

50 μl

$299
M00292-3-200ul

200 μl

$347

Free Secondary Antibody

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M00292-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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