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Product Info Summary
| SKU: | A00263 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MDA5 IFIH1 Antibody
SKU/Catalog Number
A00263
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MDA5 IFIH1 Antibody (Catalog # A00263). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
MDA5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MDA5 IFIH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00263)
Host
Rabbit
Contents
MDA5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5. The immunogen is located within the last 50 amino acids of MDA5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross response to IKKa, IKKb, IKKy, or IKKe.
Reactive Species
A00263 is reactive to IFIH1 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
116689 MW
Background of MDA5
The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00263 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1
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Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 μg/ml.
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Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.
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Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For IFIH1 (Source: Uniprot.org, NCBI)
Gene Name
IFIH1
Full Name
Interferon-induced helicase C domain-containing protein 1
Weight
116689 MW
Superfamily
helicase family
Alternative Names
Hlcd, MDA5, MDA-5, RLR-2, IDDM19, RH116, Interferon-induced helicase C domain-containing protein 1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen IFIH1 AGS7, Hlcd, IDDM19, MDA-5, MDA5, RLR-2, SGMRT1 interferon induced with helicase C domain 1 interferon-induced helicase C domain-containing protein 1|CADM-140 auto|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RIG-I-like receptor 2|RNA helicase-DEAD box protein 116|clinically amyopathic dermatomyositis auto 140 kDa|helicard|helicase with 2 CARD domains|melanoma differentiation-associated gene 5|melanoma differentiation-associated protein 5|murabutide down-regulated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFIH1, check out the IFIH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFIH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MDA5 IFIH1 Antibody (A00263)
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