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Product Info Summary
| SKU: | PA1905 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MCSF Receptor/CSF1R Antibody Picoband®
View all M-CSFR/CD115 Antibodies
SKU/Catalog Number
PA1905
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCSF Receptor/CSF1R Antibody catalog # PA1905. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCSF Receptor/CSF1R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1905)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MCSF Receptor, different from the related mouse sequence by one amino acid and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1905 is reactive to CSF1R in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
108 kDa
Calculated molecular weight
107984 MW
Background of M-CSFR/CD115
CSF1R (Colony-Stimulating Factor 1 Receptor) also known as MCSFR, FMS, c-FMS, CD115, ONCOGENE FMS or CD115 ANTIGEN, encodes a tyrosine kinase growth factor receptor for colony-stimulating factor-1, the macrophage-and monocyte-specific growth factor. The gene is located on long arm of chromosome 5 (5q32) on the Crick (minus) strand. The as-yet-unidentified CSF1 Rpromoter/enhancer sequences may be confined to the nucleotides separating the 2 genes or could potentially lie within the PDGFR gene itself. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. The encoded protein is atyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Kondo et al. (2000) showed that the endogenous myelomonocytic cytokine receptors for GM-CSF and macrophage colony-stimulating factor (CSF1R) are expressed at low to moderate levels on the more primitive hematopoietic stem cells, are absent on common lymphoid progenitors, and are upregulated after myeloid lineage induction by IL2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1905 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Intestine Tissue, human Raji whole cell, mouse RAW2467 whole cell
Validation Images & Assay Conditions
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Anti-MCSF Receptor antibody, PA1905, Western blotting
WB: Rat Intestine Tissue Lysate
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Figure 2. Western blot analysis of MCSF Receptor using anti-MCSF Receptor antibody (PA1905).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates
Lane 2: mouse RAW246.7 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCSF Receptor antigen affinity purified polyclonal antibody (Catalog # PA1905) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCSF Receptor at approximately 108KD. The expected band size for MCSF Receptor is at 108KD.
Protein Target Info & Infographic
Gene/Protein Information For CSF1R (Source: Uniprot.org, NCBI)
Gene Name
CSF1R
Full Name
Macrophage colony-stimulating factor 1 receptor
Weight
107984 MW
Superfamily
protein kinase superfamily
Alternative Names
Macrophage colony-stimulating factor 1 receptor;CSF-1 receptor;CSF-1-R;CSF-1R;M-CSF-R;2.7.10.1;Proto-oncogene c-Fms;CD115;CSF1R;FMS; CSF1R BANDDOS, C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, HDLS, M-CSF-R colony stimulating factor 1 receptor macrophage colony-stimulating factor 1 receptor|CD115 |CSF-1 receptor|FMS proto-oncogene|McDonough feline sarcoma viral (v-fms) oncogene homolog|macrophage colony stimulating factor I receptor|proto-oncogene c-Fms
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSF1R, check out the CSF1R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSF1R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCSF Receptor/CSF1R Antibody Picoband® (PA1905)
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No publications found for PA1905
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Customer Q&As
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16 Customer Q&As for Anti-MCSF Receptor/CSF1R Antibody Picoband®
Question
My question regarding product PA1905, anti-MCSF Receptor/CSF1R antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1905 anti-MCSF Receptor/CSF1R antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-16
Question
Will PA1905 anti-MCSF Receptor/CSF1R antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
As indicated on the product datasheet, PA1905 anti-MCSF Receptor/CSF1R antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-12
Question
you antibody using your anti-MCSF Receptor/CSF1R antibody for microglial cell proliferation studies. Has this antibody been tested with western blotting on rat intestine tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
We appreciate your inquiry. This PA1905 anti-MCSF Receptor/CSF1R antibody is validated on rat intestine tissue, tissue lysate, human raji whole cell lysates. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-19
Question
I see that the anti-MCSF Receptor/CSF1R antibody PA1905 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-MCSF Receptor/CSF1R antibody PA1905. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-15
Question
Do you have a BSA free version of anti-MCSF Receptor/CSF1R antibody PA1905 available?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MCSF Receptor/CSF1R antibody PA1905 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-23
Question
I was wanting to use your anti-MCSF Receptor/CSF1R antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
It shows on the product datasheet, PA1905 anti-MCSF Receptor/CSF1R antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-08
Question
My colleagues were satisfied with the WB result of your anti-MCSF Receptor/CSF1R antibody. However we have observed positive staining in placenta cell membrane using this antibody. Is that expected? Could you tell me where is CSF1R supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
According to literature, placenta does express CSF1R. Generally CSF1R expresses in cell membrane. Regarding which tissues have CSF1R expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 4028159, 9027509
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-07-29
Question
We have observed staining in human plasma. Any tips? Is anti-MCSF Receptor/CSF1R antibody supposed to stain plasma positively?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
From what I have seen in literature plasma does express CSF1R. From what I have seen in Uniprot.org, CSF1R is expressed in spleen, placenta, brain, plasma, liver, among other tissues. Regarding which tissues have CSF1R expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 4028159, 9027509
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-07-19
Question
Would anti-MCSF Receptor/CSF1R antibody PA1905 work on canine WB with liver?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
Our lab technicians have not tested anti-MCSF Receptor/CSF1R antibody PA1905 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MCSF Receptor/CSF1R antibody PA1905 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-09
Question
I am interested in to test anti-MCSF Receptor/CSF1R antibody PA1905 on rat liver for research purposes, then I may be interested in using anti-MCSF Receptor/CSF1R antibody PA1905 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The products we sell, including anti-MCSF Receptor/CSF1R antibody PA1905, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-14
Question
Is a blocking peptide available for product anti-MCSF Receptor/CSF1R antibody (PA1905)?
Verified Customer
Verified customer
Asked: 2019-01-22
Answer
We do provide the blocking peptide for product anti-MCSF Receptor/CSF1R antibody (PA1905). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-22
Question
We are currently using anti-MCSF Receptor/CSF1R antibody PA1905 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
The anti-MCSF Receptor/CSF1R antibody (PA1905) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-02
Question
I have attached the WB image, lot number and protocol we used for liver using anti-MCSF Receptor/CSF1R antibody PA1905. Please let me know if you require anything else.
A. Jones
Verified customer
Asked: 2015-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-29
Question
Would anti-MCSF Receptor/CSF1R antibody PA1905 work for WB with liver?
D. Johnson
Verified customer
Asked: 2014-06-18
Answer
According to the expression profile of liver, CSF1R is highly expressed in liver. So, it is likely that anti-MCSF Receptor/CSF1R antibody PA1905 will work for WB with liver.
Boster Scientific Support
Answered: 2014-06-18
Question
Is this PA1905 anti-MCSF Receptor/CSF1R antibody reactive to the isotypes of CSF1R?
K. Mangal
Verified customer
Asked: 2013-12-20
Answer
The immunogen of PA1905 anti-MCSF Receptor/CSF1R antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human MCSF Receptor(955-972aa EQGDIAQPLLQPNNYQFC), different from the related mouse sequence by one amino acid and from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-12-20
