CSFR (Phospho-Tyr561) Colorimetric Cell-Based ELISA Kit

M-CSFR/CD115 ELISA kit, phospho-specific, cell-based

The CSFR (Phospho-Tyr561) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CSFR (Phospho-Tyr561) protein expression profile in cells. The kit can be used for measuring the relative amounts of CSFR (Phospho-Tyr561) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CSFR (Phospho-Tyr561).

Product Info Summary

SKU: EKC2238
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

CSFR (Phospho-Tyr561) Colorimetric Cell-Based ELISA Kit

See all M-CSFR/CD115 products

SKU/Catalog Number

EKC2238

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The CSFR (Phospho-Tyr561) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CSFR (Phospho-Tyr561) protein expression profile in cells. The kit can be used for measuring the relative amounts of CSFR (Phospho-Tyr561) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CSFR (Phospho-Tyr561).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

CSFR (Phospho-Tyr561) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2238)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2238 is reactive to CSF1R in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2238 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-CSFR Phospho-Tyr561 Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-CSFR Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For CSF1R (Source: Uniprot.Org, NCBI)

Gene Name

CSF1R

Full Name

Macrophage colony-stimulating factor 1 receptor

Weight

107984 MW

Superfamily

protein kinase superfamily

Alternative Names

c-fms;CD115;CSF-1-R;CSF1R;CSFMR;FMS;Fms proto-oncogene;kinase CSFR;M-CSFR;Macrophage colony stimulating factor I receptor precursor CSF1R BANDDOS, C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, HDLS, M-CSF-R colony stimulating factor 1 receptor macrophage colony-stimulating factor 1 receptor|CD115 |CSF-1 receptor|FMS proto-oncogene|McDonough feline sarcoma viral (v-fms) oncogene homolog|macrophage colony stimulating factor I receptor|proto-oncogene c-Fms

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CSF1R, check out the CSF1R Infographic

CSF1R infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CSF1R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Loading publications

No publications found

Do you have publications using this product? Share with us and receive a reward. Contact us for more information.

Publications not available. Please try again later.

Product has been cited in publications

No results found matching your query

  • Published: --- Journal: --- Application: --- Impact Factor: --- Species: --- PMID: None

Have you used CSFR (Phospho-Tyr561) Colorimetric Cell-Based ELISA Kit?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For CSFR (Phospho-Tyr561) Colorimetric Cell-Based ELISA Kit

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for CSFR (Phospho-Tyr561) Colorimetric Cell-Based ELISA Kit

$540
Backordered.

Lead time for this item is typically 5-7 days

Get A Quote
In stock
Order Product
EKC2238
$540.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.