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Product Info Summary
| SKU: | PB9570 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MCP1/CCL2 Antibody Picoband®
SKU/Catalog Number
PB9570
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCP1/CCL2 Antibody Picoband® catalog # PB9570. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCP1/CCL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9570)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MCP-1 recombinant protein (Position: Q24-T99). Human MCP-1 shares 60.9% and 59.4% amino acid (aa) sequence identity with mouse and rat MCP-1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9570 is reactive to CCL2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated molecular weight
11025 MW
Background of CCL2
Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9570 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: SW620 Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MCP-1 using anti-MCP-1 antibody (PB9570).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: SW620 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP-1 antigen affinity purified polyclonal antibody (Catalog # PB9570) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP-1 at approximately 11 kDa. The expected band size for MCP-1 is at 11 kDa.
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Figure 2. IHC analysis of MCP1/CCL2 using anti-MCP1/CCL2 antibody (PB9570).
MCP1/CCL2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCP1/CCL2 Antibody (PB9570) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of MCP1/CCL2 using anti-MCP1/CCL2 antibody (PB9570).
MCP1/CCL2 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCP1/CCL2 Antibody (PB9570) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CCL2 (Source: Uniprot.org, NCBI)
Gene Name
CCL2
Full Name
C-C motif chemokine 2
Weight
11025 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 2;HC11;Monocyte chemoattractant protein 1;Monocyte chemotactic and activating factor;MCAF;Monocyte chemotactic protein 1;MCP-1;Monocyte secretory protein JE;Small-inducible cytokine A2;CCL2;MCP1, SCYA2; CCL2 GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF C-C motif chemokine ligand 2 C-C motif chemokine 2|chemokine (C-C motif) ligand 2|monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte chemotactic protein 1|monocyte secretory protein JE|small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|small inducible cytokine subfamily A (Cys-Cys), member 2|small-inducible cytokine A2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCL2, check out the CCL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCP1/CCL2 Antibody Picoband® (PB9570)
Hello CJ!
PB9570 has been cited in 25 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of chemokines CCL5 and CCL11 by smooth muscle tumor cells of the uterus and its possible role in the recruitment of mast cells
Salusin-%u03B2 not salusin-%u03B1 promotes vascular inflammation in ApoE-deficient mice via the I-%u03BAB%u03B1/NF-%u03BAB pathway
Comparison of biomarkers in rat renal ischemia-reperfusion injury
Salusin-?, but Not Salusin-?, Promotes Human Umbilical Vein Endothelial Cell Inflammation via the p38 MAPK/JNK-NF-?B Pathway
Bindarit reduces the incidence of acute aortic dissection complicated lung injury via modulating NF-?B pathway
Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm
Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats
Dynamic expressions of monocyte chemo attractant protein-1 and CC chamomile receptor 2 after balloon injury and their effects in intimal proliferation
Attenuation of atherosclerotic lesions in diabetic apolipoprotein E-deficient mice using gene silencing of macrophage migration inhibitory factor
Fluoxetine inhibited extracellular matrix of pulmonary artery and inflammation of lungs in monocrotaline-treated rats
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-MCP1/CCL2 Antibody Picoband®
Question
I purchased your product PB9570 and wondering how many MW (kDa) does this antibody show in Western blot using MCP-1 positive cell lysate?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The antibody PB9570 was raised against human MCP-1 thus, the band you will be able to see at 8.6 kDa on Western Blot.
Boster Scientific Support
Answered: 2019-12-12
Question
We have observed staining in human pancreas. Do you have any suggestions? Is anti-MCP1/CCL2 antibody supposed to stain pancreas positively?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
Based on literature pancreas does express CCL2. Based on Uniprot.org, CCL2 is expressed in smooth muscle tissue, glial tumor, urinary bladder, pancreas, among other tissues. Regarding which tissues have CCL2 expression, here are a few articles citing expression in various tissues:
Glial tumor, Pubmed ID: 2465924
Pancreas, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-21
Question
We purchased anti-MCP1/CCL2 antibody for IHC-P on glial tumor in the past. I am using human, and I plan to use the antibody for WB next. My question regards examining glial tumor as well as smooth muscle tissue in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
I took a look at the website and datasheets of our anti-MCP1/CCL2 antibody and it appears that PB9570 has been validated on human in both IHC-P and WB. Thus PB9570 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-14
Question
My boss were content with the WB result of your anti-MCP1/CCL2 antibody. However we have seen positive staining in smooth muscle tissue secreted using this antibody. Is that expected? Could you tell me where is CCL2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
According to literature, smooth muscle tissue does express CCL2. Generally CCL2 expresses in secreted. Regarding which tissues have CCL2 expression, here are a few articles citing expression in various tissues:
Glial tumor, Pubmed ID: 2465924
Pancreas, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-05-01
Question
My lab would like using your anti-MCP1/CCL2 antibody for lymphocyte chemotaxis studies. Has this antibody been tested with western blotting on sw620 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-12-07
Answer
Thanks for your inquiry. This PB9570 anti-MCP1/CCL2 antibody is tested on sw620 whole cell lysate. It is guaranteed to work for ELISA, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-12-07
Question
We are currently using anti-MCP1/CCL2 antibody PB9570 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
V. Lewis
Verified customer
Asked: 2014-10-31
Answer
The anti-MCP1/CCL2 antibody (PB9570) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-31
