Click image to see more details
Product Info Summary
| SKU: | A01201-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-MAP2 Antibody
SKU/Catalog Number
A01201-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MAP2 Antibody (Catalog # A01201-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01201-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.1819~1823 (T-A-A-L-A) derived from Human MAP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01201-1 is reactive to MAP2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
81276 MW
Background of MAP2
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
DeGiorgis J.A., Jaffe H., Moreira J.E., Carlotti C.G. Jr. Proteome Res. 4:306-315(2005)
Olsen J.V., Blagoev B., Gnad F., Macek B. Mann M.Cell 127:635-648(2006)
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. Science 316:1160-1166(2007)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01201-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 55-75(2c/2d), 280(2a/2b)kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Western blot analysis of extract from C6 cells using MAP2 Antibody #A01201-1
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Immunofluorescence staining of paraffin-embedded Glioma cells of Spinal Cord using MAP2 Antibody #A01201-1.
Protein Target Info & Infographic
Gene/Protein Information For MAP2 (Source: Uniprot.org, NCBI)
Gene Name
MAP2
Full Name
Microtubule-associated protein 2
Weight
81276 MW
Alternative Names
Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;INCAM-100;CD106;VCAM1; MAP2 MAP-2A, MAP2B, MAP2C, MAP2 microtubule associated protein 2 microtubule-associated protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2, check out the MAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP2 Antibody (A01201-1)
Hello CJ!
A01201-1 has been cited in 19 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Glycogen synthase kinase-3β inhibitor SB216763 promotes DNA repair in ischemic retinal neurons
Tauroursodeoxycholic acid alleviates secondary injury in spinal cord injury mice by reducing oxidative stress, apoptosis, and inflammatory response
Tauroursodeoxycholic Acid Alleviates Secondary Injury in Spinal Cord Injury Mice Through Reducing Oxidative Stress
Authors:Yonghui Hou,Jiyao Luan,Tiancheng Deng et al.
Species: Mouse
A01201-1 usage in article: APP:IF, SAMPLE:CORTICAL NEURONS, DILUTION:1:200
Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Yang H,Zhang C.Expression and cellular distribution of FGF13 in cortical tubers of the tuberous sclerosis complex.Neurosci Lett. 2021 Feb 11:135714.doi:10.1016/j.neulet.2021.135714.Epub ahead of print.PMID:33582188.
Species: Human
A01201-1 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:50
Yu Q,Li X,Li Y,Fu J,Xiao Z.Effects of combined electroacupuncture and exercise training on motor function and microtubule-associated protein 2 expression in the middle and late stages of cerebral infarction in rats.Acupunct Med.2020 Jun;38(3):175-180.doi:
Species: Rat
A01201-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:100
Neural stem cells over-expressing brain-derived neurotrophic factor promote neuronal survival and cytoskeletal protein expression in traumatic brain injury %u2026
Nitrobenzylthioinosine mimics adenosine to attenuate the epileptiform discharge of hippocampal neurons from epileptic rats
Association of RASgrf1 methylation with epileptic seizures
Direct reprogramming of fibroblasts into neural stem cells by single non-neural progenitor transcription factor Ptf1a
Lithium promotes DNA stability and survival of ischemic retinal neurocytes by upregulating DNA ligase IV
Recommended Resources
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-MAP2 Antibody
Question
We have observed staining in human cervix carcinoma. What should we do? Is anti-MAP2 antibody supposed to stain cervix carcinoma positively?
G. Johnson
Verified customer
Asked: 2020-03-19
Answer
According to literature cervix carcinoma does express MAP2. According to Uniprot.org, MAP2 is expressed in dorsolateral prefrontal cortex, brain, brain pancreas, cervix carcinoma, liver, among other tissues. Regarding which tissues have MAP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8294038
Brain, and Pancreas, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-03-19
Question
We are currently using anti-MAP2 antibody A01201-1 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-MAP2 antibody (A01201-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-MAP2 antibody A01201-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-15
Question
I see that the anti-MAP2 antibody A01201-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-03
Question
We ordered your anti-MAP2 antibody for IF on brain in a previous experiment. I am using human, and We are going to use the antibody for WB next. Our lab want to know about examining brain as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
I have checked the website and datasheets of our anti-MAP2 antibody and I see that A01201-1 has been tested on human in both IF and WB. Thus A01201-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-27
Question
Do you have a BSA free version of anti-MAP2 antibody A01201-1 available?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MAP2 antibody A01201-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-13
Question
I was wanting to use your anti-MAP2 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
As indicated on the product datasheet, A01201-1 anti-MAP2 antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-03
Question
Will A01201-1 anti-MAP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
You can see on the product datasheet, A01201-1 anti-MAP2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-11
Question
you antibody using your anti-MAP2 antibody for neuron projection development studies. Has this antibody been tested with western blotting on glioma cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
Thank you for your inquiry. This A01201-1 anti-MAP2 antibody is validated on glioma cells. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-04
Question
Is a blocking peptide available for product anti-MAP2 antibody (A01201-1)?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
We do provide the blocking peptide for product anti-MAP2 antibody (A01201-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-29
Question
Please see the WB image, lot number and protocol we used for brain using anti-MAP2 antibody A01201-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-29
Question
Is this A01201-1 anti-MAP2 antibody reactive to the isotypes of MAP2?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
The immunogen of A01201-1 anti-MAP2 antibody is Peptide sequence around aa.1819~1823 (T-A-A-L-A) derived from Human MAP2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-10-03
Question
We want to test anti-MAP2 antibody A01201-1 on mouse brain for research purposes, then I may be interested in using anti-MAP2 antibody A01201-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Jha
Verified customer
Asked: 2018-04-10
Answer
The products we sell, including anti-MAP2 antibody A01201-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-10
Question
Would anti-MAP2 antibody A01201-1 work for WB with brain?
J. Carter
Verified customer
Asked: 2017-11-10
Answer
According to the expression profile of brain, MAP2 is highly expressed in brain. So, it is likely that anti-MAP2 antibody A01201-1 will work for WB with brain.
Boster Scientific Support
Answered: 2017-11-10
Question
We were well pleased with the WB result of your anti-MAP2 antibody. However we have seen positive staining in brain pancreas cytoplasm using this antibody. Is that expected? Could you tell me where is MAP2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
According to literature, brain pancreas does express MAP2. Generally MAP2 expresses in cytoplasm, cytoskeleton. cell. Regarding which tissues have MAP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8294038
Brain, and Pancreas, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-09-05
Question
I have a question about product A01201-1, anti-MAP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Krishna
Verified customer
Asked: 2015-02-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01201-1 anti-MAP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-02-03
