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Product Info Summary
| SKU: | M01201-3 |
|---|---|
| Size: | 50ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Chicken |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody
SKU/Catalog Number
M01201-3
Size
50ul
Description
Boster Bio Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody catalog # M01201-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Stable at 4° for at least one year
Cite This Product
Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01201-3)
Host
Chicken
Contents
Concentrated IgY preparation, 0.02% sodium azide
Clonality
Polyclonal
Immunogen
Recombinant human construct of projection domain sequences, amino acids 235-1588
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01201-3 is reactive to MAP2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
199526 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01201-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:20,000-50,000.
IF/ICC and IHC: 1:5,000-1:10,000.
Validation Images & Assay Conditions
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Western blot analysis of whole brain tissue lysates using chicken pAb to microtubule associated protein 2 (MAP2), M01201-3, dilution 1:50,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain, [4] adult mouse brain. Strong band at ~280kDa mark corresponds to two major isoforms of MAP2 protein referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected if the antibody is used at higher concentrations.
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Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to microtubule associated protein 2 (MAP2), M01201-3, dilution 1:10,000 in red, and mouse mAb, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. M01201-3 antibody stains dendrites and perikarya of neurons, while M01201-3 antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
Protein Target Info & Infographic
Gene/Protein Information For MAP2 (Source: Uniprot.org, NCBI)
Gene Name
MAP2
Full Name
Microtubule-associated protein 2
Weight
199526 MW
Alternative Names
Microtubule-associated protein 2;MAP-2;MAP2; MAP2 MAP-2A, MAP2B, MAP2C, MAP2 microtubule associated protein 2 microtubule-associated protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2, check out the MAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody (M01201-3)
Hello CJ!
M01201-3 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Neural stem cells over-expressing brain-derived neurotrophic factor promote neuronal survival and cytoskeletal protein expression in traumatic brain injury %u2026
Nitrobenzylthioinosine mimics adenosine to attenuate the epileptiform discharge of hippocampal neurons from epileptic rats
Association of RASgrf1 methylation with epileptic seizures
Direct reprogramming of fibroblasts into neural stem cells by single non-neural progenitor transcription factor Ptf1a
Lithium promotes DNA stability and survival of ischemic retinal neurocytes by upregulating DNA ligase IV
Effects of Tetramethylpyrazine on Functional Recovery and Neuronal Dendritic Plasticity after Experimental Stroke
Brca1 Is Upregulated by 5-Aza-CdR and Promotes DNA Repair and Cell Survival, and Inhibits Neurite Outgrowth in Rat Retinal Neurons
Wang D, Ren M, Guo J, Yang G, Long X, Hu R, Shen W, Wang X, Zeng K. Plos One. 2014 Dec 23;9(12):E115801. Doi: 10.1371/Journal.Pone.0115801. Ecollection 2014. The Inhibitory Effects Of Npas4 On Seizures In Pilocarpine-Induced Epileptic Rats.
Sun X, Jiang R, Zhang Y, Chen M, Xiang P, Qi Y, Gao Q, Huang B, Ge J. Mol Vis. 2009 Dec 2;15:2503-14. Gene Expression And Differentiation Characteristics In Mice E13.5 And E17.5 Neural Retinal Progenitors.
Li T, Mo X, Jiang Z, He W, Lu W, Zhang H, Zhang J, Zeng L, Yang B, Xiao H, Hu Z. Oxid Med Cell Longev. 2012;2012:945071. Doi: 10.1155/2012/945071. Epub 2012 Oct 12. Study Of ??b-Crystallin Expression In Gerbil Bcao Model Of Transient Global Cerebr...
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Customer Q&As
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16 Customer Q&As for Anti-Chicken PAb Microtubule Associated Protein 2 MAP2 Antibody
Question
you antibody to test anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3 on rat liver for research purposes, then I may be interested in using anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The products we sell, including anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-27
Question
Will M01201-3 anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
You can see on the product datasheet, M01201-3 anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-26
Question
Would anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
According to the expression profile of liver, MAP2 is highly expressed in liver. So, it is likely that anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3 will work for IHC with liver.
Boster Scientific Support
Answered: 2020-03-06
Question
Is this M01201-3 anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody reactive to the isotypes of MAP2?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The immunogen of M01201-3 anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody is Recombinant human construct of projection domain sequences, amino acids 377-1505: Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-20
Question
I was wanting to use your anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody for IHC for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
As indicated on the product datasheet, M01201-3 anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-01
Question
We are currently using anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody (M01201-3) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-03
Question
We have seen staining in mouse brain. Any tips? Is anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Based on literature brain does express MAP2. Based on Uniprot.org, MAP2 is expressed in dorsolateral prefrontal cortex, brain, brain pancreas, cervix carcinoma, liver, among other tissues. Regarding which tissues have MAP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8294038
Brain, and Pancreas, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-08-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3. Let me know if you need anything else.
J. Gonzalez
Verified customer
Asked: 2019-04-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-11
Question
I have a question about product M01201-3, anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Krishna
Verified customer
Asked: 2019-01-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01201-3 anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-14
Question
I see that the anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-11
Question
My boss were happy with the WB result of your anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody. However we have observed positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is MAP2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
From literature, liver does express MAP2. Generally MAP2 expresses in cytoplasm, cytoskeleton. cell. Regarding which tissues have MAP2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8294038
Brain, and Pancreas, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-07-13
Question
Is there a BSA free version of anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3 available?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-20
Question
I am interested in using your anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody for negative regulation of microtubule motor activity studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Z. Rodriguez
Verified customer
Asked: 2018-04-17
Answer
I appreciate your inquiry. This M01201-3 anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody is tested on whole brain tissue, mouse brain. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-17
Question
I have attached the WB image, lot number and protocol we used for liver using anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody M01201-3. Please let me know if you require anything else.
O. Miller
Verified customer
Asked: 2017-01-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-01-16
Question
Is a blocking peptide available for product anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody (M01201-3)?
V. Moore
Verified customer
Asked: 2014-01-31
Answer
We do provide the blocking peptide for product anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody (M01201-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-01-31
Question
We purchased anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody for IHC on brain in a previous experiment. I am using human, and We intend to use the antibody for IF next. My lab would like examining brain as well as brain pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
S. Parker
Verified customer
Asked: 2013-07-30
Answer
I looked at the website and datasheets of our anti-Chicken PAb Microtubule Associated Protein 2 MAP2 antibody and it appears that M01201-3 has been validated on human in both IHC and IF. Thus M01201-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-07-30
