Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband®

Peroxiredoxin 4 antibody

Boster Bio Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband® catalog # PB9383. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9383
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband®

View all Peroxiredoxin 4 Antibodies

SKU/Catalog Number

PB9383

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband® catalog # PB9383. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9383)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 4, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9383 is reactive to PRDX4 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

28-30 kDa

Calculated molecular weight

30540 MW

Background of Peroxiredoxin 4

PRDX4 (peroxiredoxin 4) is also known as AOE37-2. The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. Functional analysis showed that PRDX4 protects glutamine synthetase from inactivation. Yeast 2-hybrid, immunoprecipitation, and immunoblot analyses indicated that PRDX4 and PRDX1 are capable of homodimerization and heterodimerization with each other but not with the mitochondrial PRDX3. Gel mobility shift and immunoblot analysis found that PRDX4 depletes NFKB binding activity together with a reduction in the amounts of p50, p65, and phosphorylated IKBA, as well as a reduction in the expression of HIV-1 viral proteins. Expression of PRDX4, alone or with PRDX1, increased the resistance of yeast cells to oxidant-induced toxicity. Jin et al. suggested PRDX4 modulates IKBA phosphorylation in the cytoplasm and thus affects a peroxiredoxin-dependent redox step.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9383 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human 293T whole cell, human A549 whole cell, human Hela whole cell, human K562 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
IHC: mouse Brain tissue, Rat Brain tissue, Human Tonsil tissue
ICC/IF: A549 cell
ICC: A549 cell
FCM: MCF-7 cell

Validation Images & Assay Conditions

Gene/Protein Information For PRDX4 (Source: Uniprot.org, NCBI)

Gene Name

PRDX4

Full Name

Peroxiredoxin-4

Weight

30540 MW

Superfamily

peroxiredoxin family

Alternative Names

Peroxiredoxin-4;1.11.1.15;Antioxidant enzyme AOE372;AOE37-2;Peroxiredoxin IV;Prx-IV;Thioredoxin peroxidase AO372;Thioredoxin-dependent peroxide reductase A0372;PRDX4; PRDX4 AOE37-2, AOE372, HEL-S-97n, PRX-4 peroxiredoxin 4 peroxiredoxin-4|antioxidant enzyme AOE372|epididymis secretory sperm binding protein Li 97n|peroxiredoxin IV|prx-IV|thioredoxin peroxidase (antioxidant enzyme)|thioredoxin peroxidase AO372|thioredoxin-dependent peroxide reductase A0372|thioredoxin-dependent peroxiredoxin 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRDX4, check out the PRDX4 Infographic

PRDX4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRDX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Peroxiredoxin 4/PRDX4 Antibody Picoband®

Question

We are currently using anti-Peroxiredoxin 4/PRDX4 antibody PB9383 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

J. Brown

Verified customer

Asked: 2020-04-13

Answer

The anti-Peroxiredoxin 4/PRDX4 antibody (PB9383) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-13

Order DetailsPrice
PB9383

100μg

$370
PB9383-10ug

10μg sample (liquid)

$99
PB9383-Biotin

100 μg Biotin conjugated

$570
PB9383-Cy3

100 μg Cy3 conjugated

$570
PB9383-Dylight488

100 μg Dylight488 conjugated

$570
PB9383-Dylight550

100 μg Dylight550 conjugated

$570
PB9383-Dylight594

100 μg Dylight594 conjugated

$570
PB9383-FITC

100 μg FITC conjugated

$570
PB9383-HRP

100 μg HRP conjugated

$570
PB9383-APC

100 μg APC conjugated

$670
PB9383-PE

100 μg PE conjugated

$670
PB9383-iFluor647

100 μg iFluor647 conjugated

$670
PB9383-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9383
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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