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Product Info Summary
| SKU: | M01543-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody
SKU/Catalog Number
M01543-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody catalog # M01543-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01543-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EOD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MAP1LC3A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01543-1 is reactive to MAP1LC3A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
18 kDa
Calculated molecular weight
14272 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01543-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human SH-SY5Y whole cell, human U251 whole cell, human SiHa whole cell, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue
IHC: human stomach tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAP1LC3A using anti-MAP1LC3A antibody (M01543-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human SiHa whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3A antigen affinity purified monoclonal antibody (Catalog # M01543-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP1LC3A at approximately 18 kDa. The expected band size for MAP1LC3A is at 14 kDa.
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Immunohistochemical analysis of paraffin-embedded human stomach, using MAP1LC3A Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Immunofluorescent analysis of Hela cells, using MAP1LC3A Antibody .
Protein Target Info & Infographic
Gene/Protein Information For MAP1LC3A (Source: Uniprot.org, NCBI)
Gene Name
MAP1LC3A
Full Name
Microtubule-associated proteins 1A/1B light chain 3A
Weight
14272 MW
Superfamily
ATG8 family
Alternative Names
Microtubule-associated proteins 1A/1B light chain 3A;Autophagy-related protein LC3 A;Autophagy-related ubiquitin-like modifier LC3 A;MAP1 light chain 3-like protein 1;MAP1A/MAP1B light chain 3 A;MAP1A/MAP1B LC3 A;Microtubule-associated protein 1 light chain 3 alpha;MAP1LC3A; Map1lc3a|1010001H21Rik, 4922501H04Rik, LC, LC3, LC3a|microtubule-associated protein 1 light chain 3 alpha|microtubule-associated proteins 1A/1B light chain 3A|MAP1 light chain 3-like protein 1|MAP1A/MAP1B LC3 A|MAP1A/MAP1B light chain 3 A|autophagy-related protein LC3 A|autophagy-related ubiquitin-like modifier LC3 A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP1LC3A, check out the MAP1LC3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP1LC3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP1LC3A/Lc3A Rabbit Monoclonal Antibody (M01543-1)
Hello CJ!
M01543-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu K,Yue J,Shen K,He J,Zhu G,Liu S,Zhang C,Yang H. Increased Expression of Fibroblast Growth Factor 13 in Cortical Lesions of the Focal Cortical Dysplasia. Brain Res Bull. 2020 Dec 4:S0361-9230(20)30710-3.doi: 10.1016/j.brainresbull.2020.11.023.Epub ahead
Species: Human
M01543-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50
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