Anti-MAP1LC3A antibody(N-term)

Product Name

Anti-MAP1LC3A antibody(N-term)

View all LC3A Antibodies

SKU/Catalog Number

M01543-2

Size

50 µl

Description

Boster Bio Anti-MAP1LC3A antibody(N-term) (Catalog # M01543-2). Tested in IHC-P-Leica, WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-MAP1LC3A antibody(N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01543-2)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-40 amino acids from human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01543-2 is reactive to Map1lc3a in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

Calculated molecular weight

14272 Da

Background of LC3A

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01543-2 is guaranteed for WB, IHC-P-Leica Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC-P-Leica: 1:500

Positive Control

Validation Images & Assay Conditions

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All lanes : Anti-MAP1LC3A antibody (N-term) at 1:500 dilution
Lane 1: Human brain tissue lysate
Lane 2: U-251 MG whole cell lysate
Lane 3: NIH/3T3 whole cell lysate
Lane 4: Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 14 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

All lanes : Anti-MAP1LC3A antibody(N-term) at 1:2000 dilution
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 14 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded human brain tissue using M01543-2 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded human heart tissue using M01543-2 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Gene/Protein Information For Map1lc3a (Source: Uniprot.org, NCBI)

Alternative Names

Apg8; APG8a; Apg8p3; ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; lc3-i, ii; MAP1A/1B light chain 3 A; MAP1ALC3; MAP1ALC3MAP1A/MAP1B LC3 A; MAP1BLC3MAP1A/MAP1B light chain 3 A; MAP1LC3A; microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A; MLP3A Map1lc3a|1010001H21Rik, 4922501H04Rik, LC, LC3, LC3a|microtubule-associated protein 1 light chain 3 alpha|microtubule-associated proteins 1A/1B light chain 3A|MAP1 light chain 3-like protein 1|MAP1A/MAP1B LC3 A|MAP1A/MAP1B light chain 3 A|autophagy-related protein LC3 A|autophagy-related ubiquitin-like modifier LC3 A

For more info on Map1lc3a, check out the Map1lc3a Infographic

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In this infographic, you will see the following information for Map1lc3a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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