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Product Info Summary
| SKU: | A01650 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Carboxypeptidase M/CPM Antibody Picoband®
View all Carboxypeptidase M Antibodies
SKU/Catalog Number
A01650
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Carboxypeptidase M/CPM Antibody Picoband® catalog # A01650. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Carboxypeptidase M/CPM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01650)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CPM, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01650 is reactive to CPM in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
50 kDa
Background of Carboxypeptidase M
CPM, mapped to 12q15, is known as Carboxypeptidase M. The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01650 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human SiHa whole cell, human Hela whole cell, human U20S whole cell
FCM: Hela cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CPM using anti-CPM antibody (A01650).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPM antigen affinity purified polyclonal antibody (Catalog # A01650) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPM at approximately 65 kDa. The expected band size for CPM is at 50 kDa.
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Figure 2. Flow Cytometry analysis of Hela cells using anti-CPM antibody (A01650).
Overlay histogram showing Hela cells stained with A01650 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPM Antibody (A01650, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CPM (Source: Uniprot.org, NCBI)
Gene Name
CPM
Full Name
Carboxypeptidase M
Weight
50 kDa
Superfamily
peptidase M14 family
Alternative Names
Carboxypeptidase M;CPM;3.4.17.12 ;CPM; CPM carboxypeptidase M carboxypeptidase M|renal carboxypeptidase|urinary carboxypeptidase B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CPM, check out the CPM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CPM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Carboxypeptidase M/CPM Antibody Picoband® (A01650)
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Customer Q&As
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1 Customer Q&As for Anti-Carboxypeptidase M/CPM Antibody Picoband®
Question
We are currently using anti-Carboxypeptidase M/CPM antibody A01650 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
D. Taylor
Verified customer
Asked: 2013-11-22
Answer
The anti-Carboxypeptidase M/CPM antibody (A01650) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-22
