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Product Info Summary
| SKU: | PA1751 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Myelin-associated glycoprotein MAG Antibody Picoband®
View all MAG/Siglec-4a Antibodies
SKU/Catalog Number
PA1751
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Myelin-associated glycoprotein MAG Antibody catalog # PA1751. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myelin-associated glycoprotein MAG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1751)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MAG, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1751 is reactive to MAG in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
69069 MW
Background of MAG/Siglec-4a
MAG (Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75 (NTR) was required for MAG-induced intracellular calcium elevation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1751 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue
IHC: mouse brain tissue, rat cerebellum tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAG using anti-MAG antibody (PA1751).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAG antigen affinity purified polyclonal antibody (Catalog # PA1751) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAG at approximately 100 kDa. The expected band size for MAG is at 69 kDa.
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Figure 2. IHC analysis of MAG using anti-MAG antibody (PA1751).
MAG was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAG Antibody (PA1751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MAG using anti-MAG antibody (PA1751).
MAG was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAG Antibody (PA1751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MAG (Source: Uniprot.org, NCBI)
Gene Name
MAG
Full Name
Myelin-associated glycoprotein
Weight
69069 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Myelin-associated glycoprotein;Siglec-4a;MAG;GMA; MAG GMA, S-MAG, SIGLEC-4A, SIGLEC4A, SPG75 myelin associated glycoprotein myelin-associated glycoprotein|sialic acid binding Ig-like lectin 4A|sialic acid-binding immunoglobulin-like lectin 4A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAG, check out the MAG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myelin-associated glycoprotein MAG Antibody Picoband® (PA1751)
Hello CJ!
PA1751 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Heterogeneity of Cortical Lesion Susceptibility Mapping in Multiple Sclerosis
Inhibition of neurite outgrowth using commercial myelin associated glycoprotein-Fc in neuro-2a cells
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Customer Reviews
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Customer Q&As
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19 Customer Q&As for Anti-Myelin-associated glycoprotein MAG Antibody Picoband®
Question
I would like using your anti-MAG antibody for negative regulation of neuron apoptotic process studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
We appreciate your inquiry. This PA1751 anti-MAG antibody is validated on rat brain tissue, tissue lysate, mouse brain, mammary cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-05
Question
Does anti-MAG antibody PA1751 work for IHC with brain {eco:0000312?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
According to the expression profile of brain {eco:0000312, MAG is highly expressed in brain {eco:0000312. So, it is likely that anti-MAG antibody PA1751 will work for IHC with brain {eco:0000312.
Boster Scientific Support
Answered: 2019-12-24
Question
Will PA1751 anti-MAG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
As indicated on the product datasheet, PA1751 anti-MAG antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-16
Question
Is a blocking peptide available for product anti-MAG antibody (PA1751)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
We do provide the blocking peptide for product anti-MAG antibody (PA1751). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain {eco:0000312 using anti-MAG antibody PA1751. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-09
Question
We have seen staining in human c1 segment of cervical spinal cord. Are there any suggestions? Is anti-MAG antibody supposed to stain c1 segment of cervical spinal cord positively?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
According to literature c1 segment of cervical spinal cord does express MAG. According to Uniprot.org, MAG is expressed in c1 segment of cervical spinal cord, brain, brain eco:0000312, embl:caa67055.1, among other tissues. Regarding which tissues have MAG expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2479762, 9495552, 14702039, 15489334
Boster Scientific Support
Answered: 2019-10-02
Question
Do you have a BSA free version of anti-MAG antibody PA1751 available?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MAG antibody PA1751 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-12
Question
I was wanting to use your anti-MAG antibody for IHC for human brain eco:0000312 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been validated and is this antibody a good choice for human brain {eco:0000312 identification?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
As indicated on the product datasheet, PA1751 anti-MAG antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain {eco:0000312 in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-08
Question
Has PA1751 been tested in IHC (frozen and FFPE sections)?
Verified customer
Asked: 2019-07-01
Answer
The Anti-Myelin-Associated Glycoprotein MAG Antibody (PA1751) has been tested for IHC-P. It has not been tested for IHC-F.
Boster Scientific Support
Answered: 2019-07-03
Question
Is this PA1751 anti-MAG antibody reactive to the isotypes of MAG?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
The immunogen of PA1751 anti-MAG antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human MAG(114-132aa KYYFRGDLGGYNQYTFSEH), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-22
Question
I see that the anti-MAG antibody PA1751 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-01
Question
See below the WB image, lot number and protocol we used for brain {eco:0000312 using anti-MAG antibody PA1751. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-03
Question
We were well pleased with the WB result of your anti-MAG antibody. However we have seen positive staining in c1 segment of cervical spinal cord cell membrane using this antibody. Is that expected? Could you tell me where is MAG supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
From literature, c1 segment of cervical spinal cord does express MAG. Generally MAG expresses in cell membrane. Regarding which tissues have MAG expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2479762, 9495552, 14702039, 15489334
Boster Scientific Support
Answered: 2018-05-23
Question
Will anti-MAG antibody PA1751 work on bovine WB with brain {eco:0000312?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
Our lab technicians have not validated anti-MAG antibody PA1751 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-MAG antibody PA1751 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain {eco:0000312 in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-13
Question
Dear Boster: I have some few question about your product Anti-MAG Antibody (PA1751). Would this antibody recognize both S and L MAG? Would it be appropriate to use on mouse CNS tissue for WB? Would it recognize deglycosylated forms of MAG?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
The product Anti-MAG Antibody (PA1751) have been tested and validated to work with mouse samples in Western Blot. Unfortunately, we do not have any data whether this antibody recognize S- or/and L-MAG as this has not been tested. Also we do not have any information about the difference between S- and L-MAG, but as the theory suggest that if it contain the sequence used to generate the antibody, then it should be able to be detected, again we cannot guarantee as it has not been tested the same also for deglycosylated MAG.
Boster Scientific Support
Answered: 2017-06-19
Question
you antibody to test anti-MAG antibody PA1751 on human brain {eco:0000312 for research purposes, then I may be interested in using anti-MAG antibody PA1751 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Brown
Verified customer
Asked: 2017-03-09
Answer
The products we sell, including anti-MAG antibody PA1751, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-03-09
Question
Can you help my question with product PA1751, anti-MAG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Li
Verified customer
Asked: 2016-08-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1751 anti-MAG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-04
Question
We ordered your anti-MAG antibody for WB on c1 segment of cervical spinal cord a few years ago. I am using mouse, and We intend to use the antibody for IHC next. I was wanting to use examining c1 segment of cervical spinal cord as well as brain eco:0000312 in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
M. Thomas
Verified customer
Asked: 2014-05-29
Answer
I took a look at the website and datasheets of our anti-MAG antibody and it appears that PA1751 has been tested on mouse in both WB and IHC. Thus PA1751 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-05-29
Question
We are currently using anti-MAG antibody PA1751 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
A. Zhang
Verified customer
Asked: 2013-07-19
Answer
The anti-MAG antibody (PA1751) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-07-19
