Anti-MAG Picoband® Antibody (monoclonal, 2G11)

MAG/Siglec-4a antibody

Boster Bio Anti-MAG Picoband® Antibody (monoclonal, 2G11) catalog # M03019. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M03019
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, WB

Product Name

Anti-MAG Picoband® Antibody (monoclonal, 2G11)

View all MAG/Siglec-4a Antibodies

SKU/Catalog Number

M03019

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MAG Picoband® Antibody (monoclonal, 2G11) catalog # M03019. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MAG Picoband® Antibody (monoclonal, 2G11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03019)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Monoclonal

Clone Number

2G11

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human MAG recombinant protein (Position: E34-R605).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M03019 is reactive to MAG in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

100 kDa

Calculated molecular weight

69.069kDa

Background of MAG/Siglec-4a

MAG (Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75 (NTR) was required for MAG-induced intracellular calcium elevation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03019 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse,Rat
Immunofluorescence, 5 μg/ml, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: rat brain tissue, mouse brain tissue
IHC: mouse brain tissue, rat brain tissue
IF: rat cerebellum tissue
FCM: U87 cell

Validation Images & Assay Conditions

Gene/Protein Information For MAG (Source: Uniprot.org, NCBI)

Gene Name

MAG

Full Name

Myelin-associated glycoprotein

Weight

69.069kDa

Superfamily

immunoglobulin superfamily

Alternative Names

"chromosomal protein, Nonhistone, HMG4 antibody|High mobility group (nonhistone chromosomal) protein 4 antibody|High mobility group box 3 antibody|High mobility group protein 2a antibody|High mobility group protein 4 antibody|High mobility group protein B3 antibody| High mobility group protein HMG4 antibody|HMG 4 antibody|HMG-2a antibody|HMG-4 antibody|HMG2A antibody|HMGB 3 antibody|HMGB3 antibody| HMGB3_HUMAN antibody|MGC90319 antibody|Non histone chromosomal protein antibody|Nonhistone chromosomal protein HMG4 antibody" MAG GMA, S-MAG, SIGLEC-4A, SIGLEC4A, SPG75 myelin associated glycoprotein myelin-associated glycoprotein|sialic acid binding Ig-like lectin 4A|sialic acid-binding immunoglobulin-like lectin 4A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAG, check out the MAG Infographic

MAG infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M03019

100μg

$370
M03019-10ug

10μg sample (liquid)

$99
M03019-Biotin

100 μg Biotin conjugated

$570
M03019-Cy3

100 μg Cy3 conjugated

$570
M03019-Dylight488

100 μg Dylight488 conjugated

$570
M03019-Dylight550

100 μg Dylight550 conjugated

$570
M03019-Dylight594

100 μg Dylight594 conjugated

$570
M03019-FITC

100 μg FITC conjugated

$570
M03019-HRP

100 μg HRP conjugated

$570
M03019-APC

100 μg APC conjugated

$670
M03019-PE

100 μg PE conjugated

$670
M03019-iFluor647

100 μg iFluor647 conjugated

$670
M03019-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M03019
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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