Anti-MAEA Antibody Picoband™

Product Name

Anti-MAEA Antibody Picoband™

View all EMP/MAEA Antibodies

SKU/Catalog Number

A08766-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-MAEA Antibody Picoband™ catalog # A08766-2. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MAEA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08766-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MAEA recombinant protein (Position: M1-E349).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08766-2 is reactive to MAEA in Human, Mouse, Rat

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

45 kDa

Calculated molecular weight

Background of EMP/MAEA

This gene encodes a protein that mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. The encoded protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08766-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg /1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Positive Control

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MAEA using anti-MAEA antibody (A08766-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAEA antigen affinity purified polyclonal antibody (Catalog # A08766-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAEA at approximately 45 kDa. The expected band size for MAEA is at 45 kDa.

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Figure 2. Flow Cytometry analysis of U251 cells using anti-MAEA antibody (A08766-2).
Overlay histogram showing U251 cells stained with A08766-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAEA Antibody (A08766-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For MAEA (Source: Uniprot.org, NCBI)

Alternative Names

EMLP; EMP; EMPCell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; HLC-10; Human lung cancer oncogene 10 protein; lung cancer-related protein 10; macrophage erythroblast attacher; MAEA; PIG5; proliferation-inducing gene 5 MAEA EMLP, EMP, GID9, HLC-10, P44EMLP, PIG5 macrophage erythroblast attacher, E3 ubiquitin ligase E3 ubiquitin-protein transferase MAEA|GID complex subunit 9, FYV10 homolog|cell proliferation-inducing gene 5 protein|erythroblast macrophage protein|human lung cancer oncogene 10 protein|lung cancer-related protein 10

For more info on MAEA, check out the MAEA Infographic

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In this infographic, you will see the following information for MAEA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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