Click image to see more details
Product Info Summary
| SKU: | A03159 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PIWI-L1 Antibody
View all PIWIL1/HIWI Antibodies
SKU/Catalog Number
A03159
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PIWI-L1 Antibody (Catalog # A03159). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PIWI-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PIWI-L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03159)
Host
Rabbit
Contents
PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1. The immunogen is located within amino acids 60 - 110 of PIWI-L1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of SYNPO are known to exist; this antibody will detect all three isoforms. SYNPO antibody is predicted to not cross-react with other SYNPO family member proteins.
Reactive Species
A03159 is reactive to PIWIL1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
98603 MW
Background of PIWIL1/HIWI
PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03159 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Click image to see more details
Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 μg/mL.
Click image to see more details
Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PIWIL1 (Source: Uniprot.org, NCBI)
Gene Name
PIWIL1
Full Name
Piwi-like protein 1
Weight
98603 MW
Superfamily
argonaute family
Alternative Names
HIWI, MIWI, PIWI, CT80.1, HIWI, Piwi-like protein 1 PIWIL1 CT80.1, HIWI, MIWI, PIWI piwi like RNA-mediated gene silencing 1 piwi-like protein 1|piwi homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PIWIL1, check out the PIWIL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PIWIL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIWI-L1 Antibody (A03159)
Hello CJ!
No publications found for A03159
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PIWI-L1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-PIWI-L1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
