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Product Info Summary
| SKU: | A00942-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LTBR Antibody Picoband®
View all Lymphotoxin beta R/TNFRSF3 Antibodies
SKU/Catalog Number
A00942-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-LTBR Antibody Picoband® catalog # A00942-2. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-LTBR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00942-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human LTBR recombinant protein (Position: Q31-Q404). Human LTBR shares 67% amino acid (aa) sequence identity with mouse LTBR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00942-2 is reactive to LTBR in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
55-60 kDa
Calculated molecular weight
46.709kDa
Background of Lymphotoxin beta R/TNFRSF3
LTBR(Lymphotoxin B Receptor), also called TNFCR or LT-BETA-R, is a receptor for lymphotoxin which in humans is encoded by the LTBR gene. By linkage analysis with recombinant inbred mouse strains, Nakamura et al.(1995) demonstrated that the Tnfcr locus is close to the Tnfr1 gene on mouse chromosome 6. Presumably, the human homolog is located on 12p13. Silva-Santos et al.(2005) reported that double-positive T cells regulate the differentiation of early thymocyte progenitors and gamma-delta cells by a mechanism dependent on the transcription factor ROR-gamma-t and the lymphotoxin-beta receptor. Lo et al.(2007) identified lymphotoxin and LIGHT(TNFSF14), tumor necrosis factor cytokine family members that are primarily expressed on lymphocytes, as critical regulators of key enzymes that control lipid metabolism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00942-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human Hut-78 whole cell, human K562 whole cell, human HEL whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LTBR using anti-LTBR antibody (A00942-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hut-78 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTBR antigen affinity purified polyclonal antibody (Catalog # A00942-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTBR at approximately 55-60 kDa. The expected band size for LTBR is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For LTBR (Source: Uniprot.org, NCBI)
Gene Name
LTBR
Full Name
Tumor necrosis factor receptor superfamily member 3
Weight
46.709kDa
Alternative Names
CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III LTBR D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3 lymphotoxin beta receptor tumor necrosis factor receptor superfamily member 3|lymphotoxin B receptor|lymphotoxin beta receptor (TNFR superfamily, member 3)|tumor necrosis factor C receptor|tumor necrosis factor receptor 2-related protein|tumor necrosis factor receptor type III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LTBR, check out the LTBR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LTBR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LTBR Antibody Picoband® (A00942-2)
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