Product Info Summary
SKU: | E08199 |
---|---|
Size: | 100μg/vial |
Reactive Species: | Human, Mouse |
Host: | Mouse |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-LRRC32 Antibody (Monoclonal, Plato-1), PE Conjugated
View all LRRC32/GARP Antibodies
SKU/Catalog Number
E08199
Size
100μg/vial
Form
Liquid
Description
Boster Bio Anti-LRRC32 Antibody (Monoclonal, Plato-1), PE Conjugated catalog # E08199. Tested in Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-LRRC32 Antibody (Monoclonal, Plato-1), PE Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # E08199)
Host
Mouse
Contents
Each vial contains 0.09% sodium azide.
Clonality
Monoclonal
Clone Number
Plato-1
Isotype
IgG1
Immunogen
Recombinant human LRRC32 (aa 20-627).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
E08199 is reactive to LRRC32 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
71979 MW
Background of LRRC32/GARP
Anti-IL17E recognizes IL-17E (also known as IL25). IL-17E is produced and secreted by intra-epithelial lymphocytes, lung epithelial cells, alveolar macrophages, eosinophils, basophils, NKT cells, mast cells and cells of the gastro-intestinal tract and uterus. IL-17E stimulates the production of Th2-type cytokines. IL-17E limits Th17 cell development by inducing the expression of IL-13 by dendritic cells or by inhibiting IL-23 production by macrophages. Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. A number of cytokines, including IL-17E, have been implicated as oncogenes, reflecting the importance of these proteins in cell growth and survival. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and pro-inflammatory pathologies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
E08199 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1:100, Human, Mouse
Validation Images & Assay Conditions
Click image to see more details
Flow cytometry analysis, intracellular stained, of 106 Jurkat cells using LRRC32, mAb (Plato-1) (PE conjugate) at a concentration of 10μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For LRRC32 (Source: Uniprot.org, NCBI)
Gene Name
LRRC32
Full Name
Transforming growth factor beta activator LRRC32
Weight
71979 MW
Superfamily
LRRC32/LRRC33 family
Alternative Names
Leucine-rich repeat-containing protein 32;Garpin;Glycoprotein A repetitions predominant;GARP;LRRC32;D11S833E, GARP; LRRC32 CPPRDD, D11S833E, GARP leucine rich repeat containing 32 transforming growth factor beta activator LRRC32|garpin|glycoprotein A repetitions predominant|leucine-rich repeat-containing protein 32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRC32, check out the LRRC32 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRC32: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRC32 Antibody (Monoclonal, Plato-1), PE Conjugated (E08199)
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Customer Q&As
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7 Customer Q&As for Anti-LRRC32 Antibody (Monoclonal, Plato-1), PE Conjugated
Question
Is a blocking peptide available for product anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated (E08199)?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
We do provide the blocking peptide for product anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated (E08199). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-26
Question
Can you help my question with product E08199, anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free E08199 anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-04
Question
Does E08199 anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
It shows on the product datasheet, E08199 anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-07
Question
Is this E08199 anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated reactive to the isotypes of LRRC32?
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
The immunogen of E08199 anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated is Recombinant human LRRC32 (aa 20-627). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-08
Question
Here is the WB image, lot number and protocol we used for placenta using anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated E08199. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-07-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-18
Question
you antibody to test anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated E08199 on mouse placenta for research purposes, then I may be interested in using anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated E08199 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Bhatt
Verified customer
Asked: 2017-11-08
Answer
The products we sell, including anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated E08199, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-08
Question
We are currently using anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated E08199 for mouse tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
The anti-LRRC32 antibody (Monoclonal, Plato-1), PE Conjugated (E08199) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-15