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Product Info Summary
| SKU: | A08199-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-LRRC32 Picoband® Antibody
View all LRRC32/GARP Antibodies
SKU/Catalog Number
A08199-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LRRC32 Picoband® Antibody catalog # A08199-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LRRC32 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08199-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LRRC32 recombinant protein (Position: H20-R544).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08199-1 is reactive to LRRC32 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
71.979kDa
Background of LRRC32/GARP
Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein. It is mapped to 11q13.5. This gene encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08199-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human SK-OV-3 whole cell, human PANC-1 whole cell, human 22RV1 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LRRC32 using anti-LRRC32 antibody (A08199-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SK-OV-3 whole cell lysates,
Lane 2: human PANC-1 whole cell lysates,
Lane 3: human 22RV1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC32 antigen affinity purified polyclonal antibody (Catalog # A08199-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRC32 at approximately 72KD. The expected band size for LRRC32 is at 72KD.
Protein Target Info & Infographic
Gene/Protein Information For LRRC32 (Source: Uniprot.org, NCBI)
Gene Name
LRRC32
Full Name
Transforming growth factor beta activator LRRC32
Weight
71.979kDa
Superfamily
LRRC32/LRRC33 family
Alternative Names
Transforming growth factor beta activator LRRC32; Garpin; Glycoprotein A repetitions predominant; GARP; Leucine-rich repeat-containing protein 32; LRRC32; D11S833E LRRC32 CPPRDD, D11S833E, GARP leucine rich repeat containing 32 transforming growth factor beta activator LRRC32|garpin|glycoprotein A repetitions predominant|leucine-rich repeat-containing protein 32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRC32, check out the LRRC32 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRC32: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRC32 Picoband® Antibody (A08199-1)
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