Click image to see more details
Product Info Summary
| SKU: | M08199 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-LRRC32 Purified Monoclonal Antibody
View all LRRC32/GARP Antibodies
SKU/Catalog Number
M08199
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LRRC32 Purified Monoclonal Antibody (Catalog# M08199). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-LRRC32 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08199)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
GARP5
Isotype
Mouse IgG1
Immunogen
Purified human sGARP protein. The mouse monoclonal antibody GARP5 recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M08199 is reactive to LRRC32 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
71979 MW
Background of LRRC32/GARP
GARP (glycoprotein A repetitions predominant protein), also known as garpin or LRRC32 (leucin-rich repeat containing protein 32) is an approximately 80 kDa transmembrane glycoprotein detected on the surface of megakaryocytes, platelets, and activated Treg cells. It binds to the latency-associated peptide (LAP) domain of pro-TGF beta and regulates its storage and activation. The expression of GARP on Treg cells seems to be necessary for their suppressive functions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08199 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1-2 μg/ml; positive control: human thrombocytes, reducing conditions
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-LRRC32 Purified Monoclonal Antibody M08199
Western blotting analysis (reducing conditions) of GARP antigen and its degradatory products in lysate of human thrombocytes by anti-GARP monoclonal antibody (GARP5).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For LRRC32 (Source: Uniprot.org, NCBI)
Gene Name
LRRC32
Full Name
Transforming growth factor beta activator LRRC32
Weight
71979 MW
Superfamily
LRRC32/LRRC33 family
Alternative Names
GARP, D11S833E LRRC32 CPPRDD, D11S833E, GARP leucine rich repeat containing 32 transforming growth factor beta activator LRRC32|garpin|glycoprotein A repetitions predominant|leucine-rich repeat-containing protein 32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LRRC32, check out the LRRC32 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LRRC32: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LRRC32 Purified Monoclonal Antibody (M08199)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-LRRC32 Purified Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-LRRC32 Purified Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
