Anti-LRRC32 Purified Monoclonal Antibody

Product Name

Anti-LRRC32 Purified Monoclonal Antibody

View all LRRC32/GARP Antibodies

SKU/Catalog Number

M08199

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LRRC32 Purified Monoclonal Antibody (Catalog# M08199). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-LRRC32 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08199)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

GARP5

Isotype

Mouse IgG1

Immunogen

Purified human sGARP protein. The mouse monoclonal antibody GARP5 recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M08199 is reactive to LRRC32 in Human

Applications

M08199 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

71979 MW

Background of LRRC32/GARP

GARP (glycoprotein A repetitions predominant protein), also known as garpin or LRRC32 (leucin-rich repeat containing protein 32) is an approximately 80 kDa transmembrane glycoprotein detected on the surface of megakaryocytes, platelets, and activated Treg cells. It binds to the latency-associated peptide (LAP) domain of pro-TGF beta and regulates its storage and activation. The expression of GARP on Treg cells seems to be necessary for their suppressive functions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1-2 μg/ml; positive control: human thrombocytes, reducing conditions

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-LRRC32 Purified Monoclonal Antibody M08199

Western blotting analysis (reducing conditions) of GARP antigen and its degradatory products in lysate of human thrombocytes by anti-GARP monoclonal antibody (GARP5).
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For LRRC32 (Source: Uniprot.org, NCBI)

Alternative Names

D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32; LRRC32 LRRC32 CPPRDD, D11S833E, GARP leucine rich repeat containing 32 transforming growth factor beta activator LRRC32|garpin|glycoprotein A repetitions predominant|leucine-rich repeat-containing protein 32

For more info on LRRC32, check out the LRRC32 Infographic

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In this infographic, you will see the following information for LRRC32: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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