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Product Info Summary
| SKU: | PB9029 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MCP3/CCL7 Antibody Picoband®
View all CCL7/MCP-3/MARC Antibodies
SKU/Catalog Number
PB9029
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCP3/CCL7 Antibody Picoband® catalog # PB9029. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCP3/CCL7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9029)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse MCP3 recombinant protein (Position: Q24-P97). Mouse MCP3 shares 60% and 95% amino acid (aa) sequences identity with human and rat MCP3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9029 is reactive to Ccl7 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
10999 MW
Background of CCL7/MCP-3/MARC
Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, MCP3 gene is mapped to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, it has been found that matrix metalloproteinases are both effectors and regulators of the inflammatory response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9029 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, -
ELISA, 0.1-0.5μg/ml, -
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MCP3 using anti-MCP3 antibody (PB9029).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Mouse MCP3 Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP3 antigen affinity purified polyclonal antibody (Catalog # PB9029) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP3 at approximately 36KD. The expected band size for MCP3 is at 36KD.
Protein Target Info & Infographic
Gene/Protein Information For Ccl7 (Source: Uniprot.org, NCBI)
Gene Name
Ccl7
Full Name
C-C motif chemokine 7
Weight
10999 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 7;Intercrine/chemokine MARC;Monocyte chemoattractant protein 3;Monocyte chemotactic protein 3;MCP-3;Protein FIC;Small-inducible cytokine A7;Ccl7;Fic, Mcp3, Scya7; CCL7 FIC, MARC, MCP-3, MCP3, NC28, SCYA6, SCYA7 C-C motif chemokine ligand 7 C-C motif chemokine 7|chemokine (C-C motif) ligand 7|monocyte chemoattractant protein 3|monocyte chemotactic protein 3|small inducible cytokine A7 (monocyte chemotactic protein 3)|small-inducible cytokine A7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccl7, check out the Ccl7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccl7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCP3/CCL7 Antibody Picoband® (PB9029)
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No publications found for PB9029
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Customer Q&As
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1 Customer Q&As for Anti-MCP3/CCL7 Antibody Picoband®
Question
We are currently using anti-MCP3/CCL7 antibody PB9029 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The anti-MCP3/CCL7 antibody (PB9029) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-15
