Anti-Livin/Birc7 Antibody Picoband™

Product Name

Anti-Livin/Birc7 Antibody Picoband™

View all Livin Antibodies

SKU/Catalog Number

A02577-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Livin/Birc7 Antibody Picoband™ catalog # A02577-2. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Livin/Birc7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02577-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Livin/Birc7 recombinant protein (Position: Q41-S285).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02577-2 is reactive to BIRC7 in Mouse

Applications

A02577-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

33 kDa

Calculated molecular weight

Background of Livin

Baculoviral IAP repeat-containing protein 7 is a protein that in humans is encoded by the BIRC7 gene. This protein is a member of the family of inhibitor of apoptosis proteins(IAP) and contains a single copy of a baculovirus IAP repeat(BIR) and a RING finger motif. Its gene is mapped to 20q13.3. It has got 280- and 298- amino acids. The protein is highly expressed in melanomas while lowly expressed in some lymphomas, fetal kidney, fetal liver, testis and thymus. BIRC7 plays a vital role in blocking apoptosis induced.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Livin/Birc7 using anti-Livin/Birc7 antibody (A02577-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates,
Lane 2: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Livin/Birc7 antigen affinity purified polyclonal antibody (Catalog # A02577-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Livin/Birc7 at approximately 33 kDa. The expected band size for Livin/Birc7 is at 33 kDa.

Gene/Protein Information For BIRC7 (Source: Uniprot.org, NCBI)

Alternative Names

baculoviral IAP repeat containing 7; baculoviral IAP repeat-containing 7; BIRC7; KIAP; KIAPRING finger protein 50; Kidney inhibitor of apoptosis protein; livin inhibitor-of-apoptosis; Livin; Melanoma inhibitor of apoptosis protein; ML-IAP; MLIAPlivin inhibitor of apoptosis; ML-IAPmliap; RNF50LIVINbaculoviral IAP repeat-containing protein 7 BIRC7 KIAP, LIVIN, ML-IAP, MLIAP, RNF50 baculoviral IAP repeat containing 7 baculoviral IAP repeat-containing protein 7|RING finger protein 50|RING-type E3 ubiquitin transferase BIRC7|kidney inhibitor of apoptosis protein|livin inhibitor of apoptosis|melanoma inhibitor of apoptosis protein

For more info on BIRC7, check out the BIRC7 Infographic

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In this infographic, you will see the following information for BIRC7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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