Anti-LGI1 Antibody

Product Name

Anti-LGI1 Antibody

View all LGI1 Antibodies

SKU/Catalog Number

A00850

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LGI1 Antibody (Catalog # A00850). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

LGI1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LGI1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00850)

Host

Rabbit

Contents

LGI1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LGI1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human LGI1. The immunogen is located within amino acids 370 - 420 of LGI1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This LGI1 antibody is predicted to be specific to LGI1 and not recognize other LGI proteins.

Reactive Species

A00850 is reactive to LGI1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

63818 MW

Background of LGI1

The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist; this LGI1 antibody will recognize only the longer form.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00850 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LGI1 antibody can be used for the detection of LGI1 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For LGI1 (Source: Uniprot.org, NCBI)

Alternative Names

EPT, ETL1, ADLTE, ADPAEF, ADPEAF, IB1099, EPITEMPIN, EPT, UNQ775/PRO1569, Leucine-rich glioma-inactivated protein 1, Epitempin-1 LGI1 ADLTE, ADPAEF, ADPEAF, EPITEMPIN, EPT, ETL1, IB1099 leucine rich glioma inactivated 1 leucine-rich glioma-inactivated protein 1|epitempin-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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