Leptin Antibodies

Leptin (LEP)

Key player in the regulation of energy balance and body weight control. Once released into the flow, has central and peripheral effects by binding LEPR, found in several cells, which results in the activation of several major signaling pathways (PubMed:17344214, PubMed:15899045, PubMed:19688109).

In the hypothalamus, functions as an appetite-regulating component that induces a decline in food intake and an increase in energy intake by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone density and secretion of hypothalamo-pituitary-adrenal hormones. From the periphery, increases basal metabolism, affects reproductive function, modulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and influences innate and adaptive immunity (By similarity) (PubMed:8589726, PubMed:11460888, PubMed:19688109, PubMed:24340098, PubMed:25060689).

Gene Information

Human
Gene Name:	LEP
Uniprot:	P41159
Entrez:	3952

Family

Belongs to:
leptin family

Alternative Names

FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

Molecular Weight

Mass (kDA):
18.641 kDA

Genomic Context

Human
Location:	7q32.1
Sequence:	7; NC_000007.14 (128241201..128257629)

Tissue Specificity

Adipose tissue is the main source of leptin it is also produced by other peripheral tissues such as the skeletal muscle (PubMed:7789654, PubMed:16052473, PubMed:12448771). Expressed by intercalated and striated tracts of submandibular and parotid salivary gland intralobular ducts (PubMed:12448771). Detected by fundic epithelium of the gastric mucosa (PubMed:10896907). Secreted into blood and gastric juice (PubMed:10896907).

Subcellular Localizations

Secreted.

Diseases

• Obesity
• Pain
• Diabetes Mellitus
• Insulin Resistance
• Malignant Neoplasms
• Neoplasms
• Diabetes Mellitus, Non-insulin-dependent
• Rabies (disorder)
• Insulin Sensitivity
• Inflammation
• Nervousness
• Hypertensive Disease

• Mammary Neoplasms
• Neuralgia
• Malignant Neoplasm Of Breast
• Hyperglycemia
• Carcinoma
• Fatty Liver
• Dyslipidemias

Pathways

• Secretion
• Pathogenesis
• Sensitization
• Energy Homeostasis
• Translation
• Cell Proliferation
• Aging
• Glucose Homeostasis
• Localization
• Lactation
• Habituation
• Methylation
• Dna Methylation

• Menarche
• Insulin Secretion
• Angiogenesis
• Gallstone Formation
• Cell Growth
• Transport
• Immune Response

PTMs

• Phosphorylation
• Oxidation
• Cleavage
• Methylation
• Glycosylation
• Nitration

• Sumoylation
• Demethylation
• Reduction
• Amidation
• Dephosphorylation
• Iodination

Research Areas

• Cytokine Research
• Diabetes Research
• Immunology
• Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/LEP

Best Uses Of The LEP Marker

Boster Bio has developed the LEP Marker, a molecular biology tool. It helps you find the protein that you want to target. It can be utilized in a variety of species and applications. It is also available to researchers across the globe which allows researchers to publish their findings as well as claim credits for their products and even submit samples specific to species. This information is accessible to all scientists.

Boster Bio's molecular Biology techniques

Molecular Biology is a branch of science that studies structure, function and the mechanisms of individual molecules. This branch of biology is closely related to technological advancements, such as nucleic acid synthesis and protein. The list of molecular biology research is longer than the one for nucleic acid synthesis. Molecular Biology studies biological processes at the molecular level. It focuses on the modifications and synthesis proteins such as nucleic acids, nucleic acids, and other molecules.

In the language, speech, and hearing sciences molecular biology is employed. These techniques allow researchers to gain a better understanding of how genetics functions and how to apply them to diseases. Boster Bio offers many molecular biology methods built on LEP marks. They include in situ hybridization, PCR, as well as DNA extraction. LEP markers are particularly useful when conducting research on otosclerosis. This is a condition in which one gene is involved.

Identification of target protein using LEP Marker

This study evaluated the specificity and sensitivity of several LEP markers. These markers had a limited specificity and sensitivity according to univariate ROC analyses. A multivariate analysis was conducted and six markers were identified for further analysis. The result was a target protein-specific panel that had 65% sensitivity and 70% specificity. The multiprotein panel performed better than the individual markers, but it didn't have the desired particularity or sensitivity.

This technology allows simultaneous detection of multiple protein markers. It is especially useful in diagnosing cerebrovascular and cardiovascular diseases. Multiple marker detection for proteins is possible with the liquid-phase chip in the present invention. The technology of the liquid-phase chip allows for multiple protein marker detection. This technology is highly efficient and easy to use. It can also be used for many heart disease-related proteins at one time.

The target protein was expressed in cells at 37 degrees Celsius and tagged with an H-tag at the C terminus. The cells were harvested after 45 min after induction and lysed with a French press. The cells were split into outer and inner membrane fractions. These fractions were separated with SDS-PAGE. The immunodecoration process was carried out using the HA-tagged proteins. After labeling, proteins were then immunoprecipitated by using an antisera to the tag HA.

The LEP marker was examined with BC tissue lysates. These lysates contained six AN 23 PN, six AN and 46 T samples. Normal breast tissue is challenging because of the low rate of prophylactic mastectomy in India. This is why, in the current study, samples were obtained from women who had benign breast disease and clear of lesions. This method detects the presence of a targeted protein with high sensitivity.

Optimization of LEP Marker

The improvement of LEP markers is essential for studying epigenetic regulation. This research tool can be used to determine the level of expression of a protein or cell antigens that are on the surface. The Boster Microplate reader is able to detect the absorbance at around 450nm. The reader can handle samples ranging from 0.5 ul to 1 milliliter. For larger samples, multichannel pipettes are advised. Boster also provides product credits to share results.

Anti-rabbit IgG antibody can be used to detect target protein

Rabbit and mouse antibodies are key instruments in a myriad of basic research techniques as well as medical diagnostic assays. Polyclonal anti-IgG secondary antibodies can detect primary antibodies. Continuous production of anti-IgG serum is required killing or immunizing large amounts of animals. This isn't just expensive, but it also poses a significant problem for animal welfare. In addition, every new batch of serum is an inherently diverse mix of antibodies. Preadsorption must be performed on each batch of anti IgG serum to get rid of non-specific antibodies. This means that every effort must be successful.

The anti-rabbit IgG secondary antibody created by SouthernBiotech is extensively studied and specifically recognizes rabbit immunoglobulin light chains, heavy chains and fragments. Since it recognizes native rabbit IgG antibodies, this antibody is perfect for use in sandwich ELISA experiments. It decreases cross-reactivity and background when using goat anti-rabbit IgG substrate.

TP1170 can detect subclass mAbs of IgG2a. TP1170 is compatible with both IRDye 680 and IRDye 800-labeled anti-rabbit IgG antibody. Nanobodies are advantageous because they are being compatible with primary antibodies, allowing dual-color blots. Since they are pre-bound to primary antibodies, they can save considerable time.

The use of fluorescent reagents provides many advantages over the traditional ECL for protein detection. Fluorescent dyes can be utilized in multiplexes to allow for an analysis that is quantitative. Fluorescent Western Blotting also permits multiple protein detection using the same blot. For instance IgG antibodies that are fluorescent is a sensitive and precise method. The method is also easy to use.