Anti-LEDGF PSIP1 Monoclonal Antibody

Product Name

Anti-LEDGF PSIP1 Monoclonal Antibody

View all LEDGF Antibodies

SKU/Catalog Number

A01960

Size

100μl

Form

Liquid

Description

Boster Bio Anti-LEDGF PSIP1 Monoclonal Antibody catalog # A01960. Tested in IF, IHC, WB applications. This antibody reacts with Human, Monkey, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LEDGF PSIP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01960)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

11168

Isotype

IgG

Immunogen

Purified recombinant fragment of human LEDGF expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01960 is reactive to PSIP1 in Human, Monkey, Rat

Applications

A01960 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

60103 MW

Background of LEDGF

CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/ calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/ retention of hippocampus-dependent long-term memory. CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. In addition autophosphorylation of the N-terminus is required for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to a Ca(2+)/calmodulin-independent state. Most likely the kinase is inactivated by the serine/ threonine protein phosphatase 2A. CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000

Validation Images & Assay Conditions

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Western Blot (WB) analysis using LEDGF Monoclonal antibody against HepG2 (1), Jurkat (2), K562 (3), Cos7 (4), PC-12 (5), HeLa (6), and NIH/3T3 (7) cell lysate.

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Immunofluorescence (IF) analysis of NIH/3T3 cells using LEDGF Monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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Immunohistochemistry (IHC) analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using LEDGF Monoclonal antibody.

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ELISA analysis of LEDGF antibody.

Gene/Protein Information For PSIP1 (Source: Uniprot.org, NCBI)

Alternative Names

CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; DFS70; LEDGF; LEDGFDFS 70; Lens epithelium-derived growth factor; MGC74712; p52; p75; PAIP; PC4 and SFRS1 interacting protein 1; PC4 and SFRS1 interacting protein 2; PC4 and SFRS1-interacting protein; PSIP1; PSIP2; transcriptional coactivator p52/p75; Transcriptional coactivator p75/p52 PSIP1 DFS70, LEDGF, PAIP, PSIP2, p52, p75 PC4 and SFRS1 interacting protein 1 PC4 and SFRS1-interacting protein|CLL-associated antigen KW-7|dense fine speckles 70 kDa protein|lens epithelium-derived growth factor|transcriptional coactivator p52/p75

For more info on PSIP1, check out the PSIP1 Infographic

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In this infographic, you will see the following information for PSIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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