Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M00425 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Lck Monoclonal Antibody
SKU/Catalog Number
M00425
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Lck Monoclonal Antibody catalog # M00425. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lck Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00425)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADBE-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Lck The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells. Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00425 is reactive to LCK in Human
Applications
M00425 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
11 kDa
Calculated molecular weight
87713 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:80
FC 1:20
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Lck expression in Jurkat cell lysate.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For LCK (Source: Uniprot.org, NCBI)
Gene Name
LCK
Full Name
Tyrosine-protein kinase Lck
Weight
87713 MW
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.10; EC 2.7.10.2; Hck-3; Lck; Lcktkr; Leukocyte C-terminal Src kinase; LSK; LSTRA; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; p56lck; p56-LCK; pp58lck; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine-protein kinase Lck; YT16 LCK IMD22, LSK, YT16, p56lck, pp58lck LCK proto-oncogene, Src family tyrosine kinase tyrosine-protein kinase Lck|T-lymphocyte specific protein tyrosine kinase p56lck|leukocyte C-terminal Src kinase|lymphocyte cell-specific protein-tyrosine kinase|p56(LSTRA) protein-tyrosine kinase|proto-oncogene tyrosine-protein kinase LCK|t cell-specific protein-tyrosine kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LCK, check out the LCK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LCK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lck Monoclonal Antibody (M00425)
Hello CJ!
No publications found for M00425
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Lck Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Lck Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-Lck Monoclonal Antibody
Question
I was wanting to use your anti-Lck Monoclonal antibody for IP for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
As indicated on the product datasheet, M00425 anti-Lck Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-20
Question
Our lab want to know about to test anti-Lck Monoclonal antibody M00425 on human leukemic t-cell for research purposes, then I may be interested in using anti-Lck Monoclonal antibody M00425 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The products we sell, including anti-Lck Monoclonal antibody M00425, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-29
Question
Is a blocking peptide available for product anti-Lck Monoclonal antibody (M00425)?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We do provide the blocking peptide for product anti-Lck Monoclonal antibody (M00425). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-17
Question
My colleagues were content with the WB result of your anti-Lck Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is LCK supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
From what I have seen in literature, leukemic t-cell does express LCK. Generally LCK expresses in cytoplasm. Regarding which tissues have LCK expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemia, Pubmed ID: 8139546
Leukemic T-cell, Pubmed ID: 7495859, 19690332
Lymph, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 11840567, 14610046
Peripheral blood lymphocyte, Pubmed ID: 11009097, 2835736, 3489486
Boster Scientific Support
Answered: 2020-01-13
Question
We ordered your anti-Lck Monoclonal antibody for WB on leukemia last year. I am using human, and We intend to use the antibody for IF next. I was wanting to use examining leukemia as well as peripheral blood lymphocyte in our next experiment. Could give a recommendation on which antibody would work the best for IF?
S. Edwards
Verified customer
Asked: 2019-11-15
Answer
I have checked the website and datasheets of our anti-Lck Monoclonal antibody and it seems that M00425 has been validated on human in both WB and IF. Thus M00425 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-15
Question
My lab would like using your anti-Lck Monoclonal antibody for translocation of zap-70 to immunological synapse studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
I appreciate your inquiry. This M00425 anti-Lck Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IP, IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-29
Question
Is this M00425 anti-Lck Monoclonal antibody reactive to the isotypes of LCK?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The immunogen of M00425 anti-Lck Monoclonal antibody is A synthesized peptide derived from human Lck The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells. Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-01
Question
Would M00425 anti-Lck Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
It shows on the product datasheet, M00425 anti-Lck Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-03
Question
My question regarding product M00425, anti-Lck Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00425 anti-Lck Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Lck Monoclonal antibody M00425. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-29
Question
Will anti-Lck Monoclonal antibody M00425 work on primate IP with peripheral blood lymphocyte?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
Our lab technicians have not tested anti-Lck Monoclonal antibody M00425 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Lck Monoclonal antibody M00425 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate peripheral blood lymphocyte in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-24
Question
I see that the anti-Lck Monoclonal antibody M00425 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-04
Question
We are currently using anti-Lck Monoclonal antibody M00425 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
The anti-Lck Monoclonal antibody (M00425) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-26
Question
Do you have a BSA free version of anti-Lck Monoclonal antibody M00425 available?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Lck Monoclonal antibody M00425 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-30
Question
We have seen staining in human peripheral blood lymphocyte. What should we do? Is anti-Lck Monoclonal antibody supposed to stain peripheral blood lymphocyte positively?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
According to literature peripheral blood lymphocyte does express LCK. According to Uniprot.org, LCK is expressed in thymus, leukemia, leukemic t-cell, lymph, peripheral blood lymphocyte, mammary cancer, cervix carcinoma, among other tissues. Regarding which tissues have LCK expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemia, Pubmed ID: 8139546
Leukemic T-cell, Pubmed ID: 7495859, 19690332
Lymph, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 11840567, 14610046
Peripheral blood lymphocyte, Pubmed ID: 11009097, 2835736, 3489486
Boster Scientific Support
Answered: 2018-03-22
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-Lck Monoclonal antibody M00425. Please let me know if you require anything else.
L. Kulkarni
Verified customer
Asked: 2013-11-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-22
Question
Does anti-Lck Monoclonal antibody M00425 work for IP with leukemic t-cell?
N. Kulkarni
Verified customer
Asked: 2013-01-15
Answer
According to the expression profile of leukemic t-cell, LCK is highly expressed in leukemic t-cell. So, it is likely that anti-Lck Monoclonal antibody M00425 will work for IP with leukemic t-cell.
Boster Scientific Support
Answered: 2013-01-15
