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Product Info Summary
| SKU: | EKC2450 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
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Product info
Product Name
Lck (Phospho-Tyr393) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2450
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Lck (Phospho-Tyr393) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Lck (Phospho-Tyr393) protein expression profile in cells. The kit can be used for measuring the relative amounts of Lck (Phospho-Tyr393) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Lck (Phospho-Tyr393).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Lck (Phospho-Tyr393) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2450)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2450 is reactive to LCK in Human, Mouse, Rat samples
Application Guarantee
EKC2450 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-Lck Phospho-Tyr393 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-Lck Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from Jurkat cells
Protein Target Info & Infographic
Gene/Protein Information For LCK (Source: Uniprot.Org, NCBI)
Gene Name
LCK
Full Name
Tyrosine-protein kinase Lck
Weight
58001 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase Lck;2.7.10.2;Leukocyte C-terminal Src kinase;LSK;Lymphocyte cell-specific protein-tyrosine kinase;Protein YT16;Proto-oncogene Lck;T cell-specific protein-tyrosine kinase;p56-LCK;LCK; LCK IMD22, LSK, YT16, p56lck, pp58lck LCK proto-oncogene, Src family tyrosine kinase tyrosine-protein kinase Lck|T-lymphocyte specific protein tyrosine kinase p56lck|leukocyte C-terminal Src kinase|lymphocyte cell-specific protein-tyrosine kinase|p56(LSTRA) protein-tyrosine kinase|proto-oncogene tyrosine-protein kinase LCK|t cell-specific protein-tyrosine kinase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LCK, check out the LCK Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LCK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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