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Product Info Summary
| SKU: | PA1743 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD2/CD2 Antibody Picoband®
SKU/Catalog Number
PA1743
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD2/CD2 Antibody catalog # PA1743. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD2/CD2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1743)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse CD2, different from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1743 is reactive to Cd2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
38415 MW
Background of CD2
CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portionThe localization of CD2 to 1p13 was established by in situ hybridization. By Southern blotting of DNA from a panel of somatic cell hybrids, Clayton et al. (1988) assigned the CD2 gene to human chromosome 1 and murine chromosome 3.CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human CD2 gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1743 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Spleen Cell, NIH/3T3 Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-CD2 antibody, PA1743, Western blotting
Lane 1: Rat Spleen Cell Lysate
Lane 2: NIH/3T3 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For Cd2 (Source: Uniprot.org, NCBI)
Gene Name
Cd2
Full Name
T-cell surface antigen CD2
Weight
38415 MW
Alternative Names
T-cell surface antigen CD2;LFA-2;LFA-3 receptor;Lymphocyte antigen 37;Ly-37;T-cell surface antigen T11/Leu-5;CD2;Cd2;Ly-37; CD2 LFA-2, SRBC, T11 CD2 molecule T-cell surface CD2|CD2 (p50), sheep red blood cell receptor|LFA-3 receptor|T-cell surface T11/Leu-5|erythrocyte receptor|lymphocyte-function -2|rosette receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd2, check out the Cd2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD2/CD2 Antibody Picoband® (PA1743)
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No publications found for PA1743
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD2/CD2 Antibody Picoband®
Question
Does anti-CD2/CD2 antibody PA1743 work on feline WB with leukocyte?
E. Rodriguez
Verified customer
Asked: 2020-04-08
Answer
Our lab technicians have not validated anti-CD2/CD2 antibody PA1743 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CD2/CD2 antibody PA1743 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline leukocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-08
Question
Is a blocking peptide available for product anti-CD2/CD2 antibody (PA1743)?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We do provide the blocking peptide for product anti-CD2/CD2 antibody (PA1743). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-12
Question
My boss were content with the WB result of your anti-CD2/CD2 antibody. However we have observed positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
Based on literature, leukocyte does express CD2. Generally CD2 expresses in cell membrane. Regarding which tissues have CD2 expression, here are a few articles citing expression in various tissues:
Pancreas, and Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-31
Question
Does anti-CD2/CD2 antibody PA1743 work for WB with pancreas spleen?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
According to the expression profile of pancreas spleen, CD2 is highly expressed in pancreas spleen. So, it is likely that anti-CD2/CD2 antibody PA1743 will work for WB with pancreas spleen.
Boster Scientific Support
Answered: 2020-01-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas spleen using anti-CD2/CD2 antibody PA1743. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-31
Question
Is this PA1743 anti-CD2/CD2 antibody reactive to the isotypes of CD2?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The immunogen of PA1743 anti-CD2/CD2 antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse CD2(224-242aa FIFCICKRRKRNRRRKDEE), different from the related rat sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-13
Question
We have seen staining in rat leukocyte. Any tips? Is anti-CD2/CD2 antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
From what I have seen in literature leukocyte does express CD2. From what I have seen in Uniprot.org, CD2 is expressed in leukocyte, pancreas spleen, among other tissues. Regarding which tissues have CD2 expression, here are a few articles citing expression in various tissues:
Pancreas, and Spleen, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-08
Question
Does PA1743 anti-CD2/CD2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
It shows on the product datasheet, PA1743 anti-CD2/CD2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-26
Question
I was wanting to use your anti-CD2/CD2 antibody for WB for rat pancreas spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat pancreas spleen identification?
A. Jackson
Verified customer
Asked: 2018-01-12
Answer
As indicated on the product datasheet, PA1743 anti-CD2/CD2 antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pancreas spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-12
Question
Is there a BSA free version of anti-CD2/CD2 antibody PA1743 available?
Verified Customer
Verified customer
Asked: 2017-08-31
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD2/CD2 antibody PA1743 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-31
Question
I see that the anti-CD2/CD2 antibody PA1743 works with WB, what is the protocol used to produce the result images on the product page?
G. Yang
Verified customer
Asked: 2017-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-03-13
Question
We are currently using anti-CD2/CD2 antibody PA1743 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on canine tissues as well?
H. Parker
Verified customer
Asked: 2017-01-09
Answer
The anti-CD2/CD2 antibody (PA1743) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-01-09
Question
My question regarding product PA1743, anti-CD2/CD2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Krishna
Verified customer
Asked: 2016-08-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1743 anti-CD2/CD2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-08
Question
I would like to test anti-CD2/CD2 antibody PA1743 on rat pancreas spleen for research purposes, then I may be interested in using anti-CD2/CD2 antibody PA1743 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Anderson
Verified customer
Asked: 2016-05-03
Answer
The products we sell, including anti-CD2/CD2 antibody PA1743, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-05-03
Question
See below the WB image, lot number and protocol we used for pancreas spleen using anti-CD2/CD2 antibody PA1743. Please let me know if you require anything else.
L. Rodriguez
Verified customer
Asked: 2013-12-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-19
