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Product Info Summary
| SKU: | A30561 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-LASS4 Antibody
SKU/Catalog Number
A30561
Size
100ul
Form
Liquid
Description
Boster Bio Anti-LASS4 Antibody (Catalog# A30561). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LASS4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30561)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human LASS4. Immunogen sequence location: 41-90
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30561 is reactive to CERS4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
46399 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30561 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HUVEC, COLO, and Jurkat cells, using LASS4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using LASS4 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CERS4 (Source: Uniprot.org, NCBI)
Gene Name
CERS4
Full Name
Ceramide synthase 4
Weight
46399 MW
Alternative Names
CERS4;LASS4;Ceramide synthase 4;CerS4;LAG1 longevity assurance homolog 4 Cers4|2900019C14Rik, Cer, L, Lass4, Trh, Trh1|ceramide synthase 4|ceramide synthase 4|LAG1 homolog, ceramide synthase 4|LAG1 longevity assurance homolog 4|TRAM homolog 1|longevity assurance homolog 4|sphingosine N-acyltransferase CERS4|translocating chain-associating membrane protein homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CERS4, check out the CERS4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CERS4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LASS4 Antibody (A30561)
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No publications found for A30561
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Customer Q&As
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5 Customer Q&As for Anti-LASS4 Antibody
Question
My question regarding product A30561, anti-LASS4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30561 anti-LASS4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-LASS4 antibody A30561 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The anti-LASS4 antibody (A30561) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
I was wanting to use your anti-LASS4 antibody for WB for human embryo on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human embryo identification?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
It shows on the product datasheet, A30561 anti-LASS4 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embryo in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-01
Question
Does anti-LASS4 antibody A30561 work on zebrafish IHC with embryo?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Our lab technicians have not validated anti-LASS4 antibody A30561 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-LASS4 antibody A30561 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish embryo in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-07
Question
I see that the anti-LASS4 antibody A30561 works with WB, what is the protocol used to produce the result images on the product page?
H. Jones
Verified customer
Asked: 2016-05-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-05-18
