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Facts about Lysosome-associated membrane glycoprotein 1.
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Human | |
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Gene Name: | LAMP1 |
Uniprot: | P11279 |
Entrez: | 3916 |
Belongs to: |
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LAMP family |
CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1
Mass (kDA):
44.882 kDA
Human | |
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Location: | 13q34 |
Sequence: | 13; NC_000013.11 (113297239..113323672) |
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome. This protein shuttles between lysosomes, endosomes, and the plasma membrane (By similarity). Colocalizes with OSBPL1A at the late endosome (PubMed:16176980).
LAMP1 is a lysosomal marker used in IHC and ELISA. This article will explain how LAMP1 is used and its uses. This article also contains information on the LAMP1 ELISA kit, which is used to measure lysosomal content. A sample application of this lysosomal markers will be covered.
LAMP1 (lysogenic protein) is expressed by hepatocytes. Inflammation induced HCMV results is the relocalization and relocalization many cell membrane components including pUL71, pAMP1 and multivesicular bodies markers. LAMP1 is distributed in the cytosol when the infection occurs, whereas AO becomes green. This dye indicates the existence of lysosomes.
Early in the morning, the LC3-RFP vesicles are colocalized with LAMP1–GFP. However, they do not colocalize with LAMP1-GFP in the late evening or night. Both of these proteins are present in the lysosomes and are fusion partners of LAMP1.
To detect the colocalization LAMP1/RyR3, CAMs were incubated first with FITC labeled anti-LAMP1 antibodies. Next, lysosomes had to be incubated for 5 mM of fluo-4, a Ca2+ sensitive dye. To visualize colocalization between these proteins, Confocal microscopy (CM) was used.
This study shows that NH2-PS NPs are capable of causing autophagy and lysosomal destruction. To assess whether NH2-PS NPs increase autophagy and lysosomal size, MEF cells were treated with the NPs for 3 and 6 h. As a result, the NPs accumulate into the lysosomal compartments and cause dramatic lysosome expansion.
LMP is inducible by NH2-PSNPs in MEF cells. This causes both partial and full cell death. Untreated cells exhibit lysosomal staining and no rupture of the plasma membrane. Both unprocessed as well as processed cathepsin D can be detected in the cytosol. LAMP1, an important lysosomal marker, is useful in determining the level and function of dysfunctionally lysosomal substances in a cell.
IHC is a common method used in medical research and drug development. It is used in drug development to detect disease markers and to measure up or down-regulation. The process involves detecting and quantifying proteins that can be detected in a variety of tissues. This method can also detect pathogens in human tissue. These are just a few of the many uses of IHC. Let's take a look at just a few.
IHC staining must be completed before paraffin can be removed from FFPE section sections. Paraffinization incompletely obscures the target antibody, which prevents antibodies from reacting with it. Aqueous solutions containing IHC staining compounds are also repelled by paraffin's hydrophobicity. Xylene, therefore, is used in the procedure. However, this solvent is flammable, toxic, and volatile. To reduce or eliminate this danger, you should use other solvents.
IHC detects target antigens directly or through fluorescent means. The conjugation or addition of antibodies to enzymes is the key to the first. The most commonly used enzymes are horseradish peroxidase, alkaline phosphatase, and alkaline dehydrogenase. However, other enzymes can also be used. The enzymes result in the formation of insoluble colored precipitates near the antigen localization area. Although it is less quantitative than other methods this is still an excellent diagnostic tool for pathologists. You will need high-quality specific ingredients to perform this type IHC.
Preparation and fixation of samples are critical steps in the IHC process. It is crucial to preserve antigens by fixing tissue samples. This process also preserves the tissue's structure and architecture. The experimental results may be affected by the use of fixatives. To ensure the success of this method, tissue fixation must be done correctly. IHC cannot work if the sample has not been properly fixed.
The Boster bio LAMP1 marker is monoclonal and reacts with Humans, Mouses, and Rats. Boster Bio antibodies can be stored at -20°C for up to one-year. It can also freeze for up to 6 months. The antibody contains 0.05 mg thimerosal and 5 mg BSA. It is useful for immunofluorescence tests and ELISA.
The BosterBio LAMP1 anti-body is validated on multiple platforms. Researchers are able to compare it against known positive or negative samples to verify its specificity. Additionally, Boster Bio recognizes and rewards researchers who review its products and contribute to the scientific community. Boster Bio also offers product credits to early users and product reviewers, rewarding scientists around the world who are interested in immunodiagnosis.
To verify that this marker was specifically targeted for PTx, the marker was coated on ELISA plates. Then, a m11E6 antibody was added, which binds to a nonoverlapping epitope. The Boster Bio marker LAMP1 was used to detect PTx. The coating was found to be strong at binding low levels of PTx.
Boster bio's LAMP1 marker can be used in ELISA kit to detect LAMP1 concentrations in a variety biologic samples. The company supplies scientists with high-quality antibodies, as well as antibody arrays, for immunoassays. The company also offers custom orders and BeNeLux delivery services. Learn more about Boster Bio LAMP1 marker.
The anti-LAMP1 antibody is made from bovine serum and reacts with the LAMP1 protein in the human body. It can be stored at 4degC or -20degC for up to a year. The antibodies are also compatible with mouse, rat and human samples. The antibodies are stable at -20degC for a year or frozen for six months. Boster Bio's LAMP1 antibody consists of five mg BSA and 0.05mg Thimerosal. Before using the sample, it must be dilute.
PMID: 3198605 by Fukuda M., et al. Cloning of cDNAs encoding human lysosomal membrane glycoproteins, h- lamp-1 and h-lamp-2. Comparison of their deduced amino acid sequences.
PMID: 2912382 by Mane S.M., et al. Purification and characterization of human lysosomal membrane glycoproteins.
*More publications can be found for each product on its corresponding product page