Anti-L1/ORF2 Antibody

Product Name

Anti-L1/ORF2 Antibody

View all ORF2 Antibodies

SKU/Catalog Number

A19762

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-L1/ORF2 Antibody (Catalog # A19762). Tested in Dot blot, ELISA, WB applications. This antibody reacts with Human, Chimpanzee.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-L1/ORF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A19762)

Host

Chicken

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgY

Immunogen

This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH).  The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A19762 is reactive to ORF2 in Human, Chimpanzee

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL

Observed Molecular Weight

19 kDa

Calculated molecular weight

23354 MW

Background of ORF2

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A19762 is guaranteed for Dot blot, ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000
WB: 1:2,000-1_10,000

Validation Images & Assay Conditions

Gene/Protein Information For ORF2 (Source: Uniprot.org, NCBI)

Alternative Names

chicken anti-L1/ORF2, chicken anti-L1-ORF2, L1 (LINE-1), Long INterspersed Element , retrotransposon ORF2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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