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Product Info Summary
| SKU: | M01275-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Ku-80 XRCC5 Monoclonal Antibody
View all Ku80/XRCC5 Antibodies
SKU/Catalog Number
M01275-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Ku-80 XRCC5 Monoclonal Antibody catalog # M01275-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ku-80 XRCC5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01275-2)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5C5
Isotype
IgG
Immunogen
Purified recombinant fragment of human Ku-80 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01275-2 is reactive to XRCC5 in Human, Mouse
Reconstitution
Calculated molecular weight
82705 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01275-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 5. ELISA validation of XRCC5 using Anti-Ku-80 XRCC5 Monoclonal Antibody (M01275-2).
ELISA analysis of Ku-80 antibody.
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Figure 4. Flow Cytometry validation of XRCC5 using Anti-Ku-80 XRCC5 Monoclonal Antibody (M01275-2).
Flow cytometric (FCM) analysis of HeLa cells using Ku-80 Monoclonal Antibody (green) and negative control (purple).
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Figure 3. Immunofluorescent staining data of XRCC5 using Anti-Ku-80 XRCC5 Monoclonal Antibody (M01275-2).
Immunofluorescence (IF) analysis of HeLa cells using Ku-80 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Figure 2. Immunohistochemistry validation of XRCC5 using Anti-Ku-80 XRCC5 Monoclonal Antibody (M01275-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Tonsil tissues (left) and Human Colon cancer tissues (right) with DAB staining using Ku-80 Monoclonal Antibody.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Ku-80 XRCC5 Monoclonal Antibody M01275-2
Western Blot (WB) analysis using Ku-80 Monoclonal Antibody against HeLa (1)Protein Target Info & Infographic
Gene/Protein Information For XRCC5 (Source: Uniprot.org, NCBI)
Gene Name
XRCC5
Full Name
X-ray repair cross-complementing protein 5
Weight
82705 MW
Superfamily
ku80 family
Alternative Names
X-ray repair cross-complementing protein 5;3.6.4.-;86 kDa subunit of Ku antigen;ATP-dependent DNA helicase 2 subunit 2;ATP-dependent DNA helicase II 80 kDa subunit;CTC box-binding factor 85 kDa subunit;CTC85;CTCBF;DNA repair protein XRCC5;Ku80;Ku86;Lupus Ku autoantigen protein p86;Nuclear factor IV;Thyroid-lupus autoantigen;TLAA;X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining);XRCC5;G22P2; XRCC5 KARP-1, KARP1, KU80, KUB2, Ku86, NFIV X-ray repair cross complementing 5 X-ray repair cross-complementing protein 5|86 kDa subunit of Ku |ATP-dependent DNA helicase 2 subunit 2|ATP-dependent DNA helicase II 80 kDa subunit|CTC box-binding factor 85 kDa subunit|CTC85|CTCBF|DNA repair protein XRCC5|Ku auto, 80kDa|Ku86 auto related protein 1|TLAA|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|lupus Ku auto protein p86|nuclear factor IV|thyroid-lupus auto
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XRCC5, check out the XRCC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XRCC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ku-80 XRCC5 Monoclonal Antibody (M01275-2)
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