Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | PB9464 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Ku80/XRCC5 Antibody Picoband®
View all Ku80/XRCC5 Antibodies
SKU/Catalog Number
PB9464
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ku80/XRCC5 Antibody Picoband® catalog # PB9464. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ku80/XRCC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9464)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Ku80, different from the related mouse sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9464 is reactive to XRCC5 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
83 kDa
Calculated molecular weight
82705 MW
Background of Ku80/XRCC5
XRCC5 (X-ray Repair,Complementing Defective ,In Chinese Hamster, 5), also known as Ku80 or Ku86, is a protein that in humans, is encoded by the XRCC5 gene. The XRCC5 gene encodes the 80-kD subunit of the Ku autoantigen, a heterodimer which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end joining (NHEJ) pathway. The XRCC5 gene is mapped to 2q35. Human colon cancer cells heterozygous for Ku86 are haploinsufficient with an increase in polyploid cells, a reduction in cell proliferation, elevated p53 levels, and a slight hypersensitivity to ionizing radiation. Functional inactivation of the second Ku86 allele results in cells with a drastically reduced doubling time. The Ku86 locus is essential in human somatic tissue culture cells by experiments demonstration. A rare microsatellite polymorphism in XRCC5 is associated with cancer in patients of varying radiosensitivity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9464 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human A549 whole cell, human 293T whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue
IHC: human lung cancer tissue
IHC-F: human placenta tissue
ICC/IF: U20S cell
FCM: SiHa cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of KU80 using anti-KU80 antibody (PB9464).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KU80 antigen affinity purified polyclonal antibody (Catalog # PB9464) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKU80 at approximately 83 kDa. The expected band size for KU80 is at 83 kDa.
Click image to see more details
Figure 2. IHC analysis of KU80 using anti-KU80 antibody (PB9464).
KU80 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KU80 Antibody (PB9464) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Ku80 using anti-Ku80 antibody (PB9464).
Ku80 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ku80 Antibody (PB9464) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of Ku80 using anti-Ku80 antibody (PB9464).
Ku80 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Ku80 Antibody (PB9464) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of SiHa cells using anti-Ku80 antibody (PB9464).
Overlay histogram showing SiHa cells stained with PB9464 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ku80 Antibody (PB9464,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For XRCC5 (Source: Uniprot.org, NCBI)
Gene Name
XRCC5
Full Name
X-ray repair cross-complementing protein 5
Weight
82705 MW
Superfamily
ku80 family
Alternative Names
X-ray repair cross-complementing protein 5;3.6.4.-;86 kDa subunit of Ku antigen;ATP-dependent DNA helicase 2 subunit 2;ATP-dependent DNA helicase II 80 kDa subunit;CTC box-binding factor 85 kDa subunit;CTC85;CTCBF;DNA repair protein XRCC5;Ku80;Ku86;Lupus Ku autoantigen protein p86;Nuclear factor IV;Thyroid-lupus autoantigen;TLAA;X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining);XRCC5;G22P2; XRCC5 KARP-1, KARP1, KU80, KUB2, Ku86, NFIV X-ray repair cross complementing 5 X-ray repair cross-complementing protein 5|86 kDa subunit of Ku |ATP-dependent DNA helicase 2 subunit 2|ATP-dependent DNA helicase II 80 kDa subunit|CTC box-binding factor 85 kDa subunit|CTC85|CTCBF|DNA repair protein XRCC5|Ku auto, 80kDa|Ku86 auto related protein 1|TLAA|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|lupus Ku auto protein p86|nuclear factor IV|thyroid-lupus auto
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XRCC5, check out the XRCC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XRCC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ku80/XRCC5 Antibody Picoband® (PB9464)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Ku80/XRCC5 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Ku80/XRCC5 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-Ku80/XRCC5 Antibody Picoband®
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Ku80/XRCC5 antibody PB9464. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-06
Question
I am interested in using your anti-Ku80/XRCC5 antibody for establishment of integrated proviral latency studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
I appreciate your inquiry. This PB9464 anti-Ku80/XRCC5 antibody is validated on rat thymus tissue, testis tissue, tissue lysate, hela whole cell lysate, nih3t3 whole cell lysate, lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-25
Question
I was wanting to use your anti-Ku80/XRCC5 antibody for IHC for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
You can see on the product datasheet, PB9464 anti-Ku80/XRCC5 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-09
Question
We are currently using anti-Ku80/XRCC5 antibody PB9464 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The anti-Ku80/XRCC5 antibody (PB9464) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-28
Question
See below the WB image, lot number and protocol we used for leukemic t-cell using anti-Ku80/XRCC5 antibody PB9464. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
Will anti-Ku80/XRCC5 antibody PB9464 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
According to the expression profile of leukemic t-cell, XRCC5 is highly expressed in leukemic t-cell. So, it is likely that anti-Ku80/XRCC5 antibody PB9464 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-08-13
Question
I would like to test anti-Ku80/XRCC5 antibody PB9464 on mouse leukemic t-cell for research purposes, then I may be interested in using anti-Ku80/XRCC5 antibody PB9464 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
The products we sell, including anti-Ku80/XRCC5 antibody PB9464, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-25
Question
I see that the anti-Ku80/XRCC5 antibody PB9464 works with IHC, what is the protocol used to produce the result images on the product page?
C. Krishna
Verified customer
Asked: 2019-04-22
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-22
Question
Our lab used your anti-Ku80/XRCC5 antibody for IHC on kidney in the past. I am using rat, and We want to use the antibody for WB next. We want examining kidney as well as thyroid uterus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
I looked at the website and datasheets of our anti-Ku80/XRCC5 antibody and it seems that PB9464 has been validated on rat in both IHC and WB. Thus PB9464 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-04-18
Question
Do you have a BSA free version of anti-Ku80/XRCC5 antibody PB9464 available?
G. Krishna
Verified customer
Asked: 2018-08-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ku80/XRCC5 antibody PB9464 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-03
Question
Is a blocking peptide available for product anti-Ku80/XRCC5 antibody (PB9464)?
P. Dhar
Verified customer
Asked: 2018-06-12
Answer
We do provide the blocking peptide for product anti-Ku80/XRCC5 antibody (PB9464). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-12
Question
We have observed staining in mouse liver. Do you have any suggestions? Is anti-Ku80/XRCC5 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
From literature liver does express XRCC5. From Uniprot.org, XRCC5 is expressed in kidney, neuroblastoma, coronary artery, thyroid uterus, cervix carcinoma, heart osteoblast, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have XRCC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8605992
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, and Osteoblast, Pubmed ID: 12145306
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Neuroblastoma, Pubmed ID: 14702039
Thyroid, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-25
Question
Will PB9464 anti-Ku80/XRCC5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Moore
Verified customer
Asked: 2017-03-30
Answer
It shows on the product datasheet, PB9464 anti-Ku80/XRCC5 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-03-30
Question
My question regarding product PB9464, anti-Ku80/XRCC5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
W. Krishna
Verified customer
Asked: 2016-12-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9464 anti-Ku80/XRCC5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-12-09
Question
My colleagues were well pleased with the WB result of your anti-Ku80/XRCC5 antibody. However we have observed positive staining in leukemic t-cell nucleus. using this antibody. Is that expected? Could you tell me where is XRCC5 supposed to be expressed?
S. Roberts
Verified customer
Asked: 2014-08-27
Answer
From what I have seen in literature, leukemic t-cell does express XRCC5. Generally XRCC5 expresses in nucleus. Regarding which tissues have XRCC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 8605992
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Heart, and Osteoblast, Pubmed ID: 12145306
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Neuroblastoma, Pubmed ID: 14702039
Thyroid, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-08-27
Question
Will anti-Ku80/XRCC5 antibody PB9464 work on pig WB with liver?
R. Jackson
Verified customer
Asked: 2013-02-13
Answer
Our lab technicians have not validated anti-Ku80/XRCC5 antibody PB9464 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Ku80/XRCC5 antibody PB9464 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-02-13
Question
Is this PB9464 anti-Ku80/XRCC5 antibody reactive to the isotypes of XRCC5?
K. Bhatt
Verified customer
Asked: 2013-02-08
Answer
The immunogen of PB9464 anti-Ku80/XRCC5 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Ku80 (649-676 aa FSEEQRFNNFLKALQEKVEIKQLNHFWE), different from the related mouse sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-02-08
