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Product Info Summary
| SKU: | EK0858 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse TNFSF4/OX40L ELISA Kit PicoKine®
View all OX40 Ligand/TNFSF4 ELISA kits
SKU/Catalog Number
EK0858
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TNFSF4/OX40L ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfsf4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNFSF4/OX40L ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0858)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S51-L198
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0858 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0858 is reactive to Tnfsf4 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0858 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of OX40 Ligand/TNFSF4
OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, Baum et al. (1994) mapped the mouse Tnfsf4 gene to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene.OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0858-CAP | Anti-Mouse Tnfsf4 Pre-coated 96-well strip microplate | 1 |
| EK0858-ST | Mouse Tnfsf4 Standard | 2 vials, 10 ng/tube |
| EK0858-DA | Mouse Tnfsf4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.034 | 0.253 | 0.367 | 0.552 | 1.010 | 1.522 | 2.060 | 2.486 |
Data Example Images
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Mouse TNFSF4/OX40L PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect OX40 Ligand/TNFSF4, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 62.5 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TNFSF4/OX40L ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 176 | 562 | 2082 | 160 | 512 | 2260 |
| Standard deviation | 10.38 | 36.81 | 141.57 | 10.88 | 38.91 | 187.58 |
| CV (%) | 6.8% | 6.8% | 6.8% | 6.8% | 7.6% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of OX40 Ligand/TNFSF4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 176 | 174 | 151 | 149 | 162 | 12.53 | 7.7% |
| Sample 2 | 562 | 596 | 510 | 588 | 564 | 33.61 | 5.9% |
| Sample 3 | 2082 | 1768 | 1972 | 2071 | 1973 | 126 | 6.3% |
Protein Target Info & Infographic
Gene/Protein Information For Tnfsf4 (Source: Uniprot.Org, NCBI)
Gene Name
Tnfsf4
Full Name
Tumor necrosis factor ligand superfamily member 4
Weight
22255 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 4;OX40 ligand;OX40L;CD252;Tnfsf4;Ox40l, Txgp1l; TNFSF4 CD134L, CD252, GP34, OX-40L, OX4OL, TNLG2B, TXGP1 TNF superfamily member 4 tumor necrosis factor ligand superfamily member 4|CD134 ligand|OX40 ligand|glycoprotein Gp34|tax-transcriptionally activated glycoprotein 1 (34kD)|tumor necrosis factor (ligand) superfamily member 4|tumor necrosis factor ligand 2B|tumor necrosis factor superfamily member 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tnfsf4, check out the Tnfsf4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfsf4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TNFSF4/OX40L ELISA Kit PicoKine® (EK0858)
Hello CJ!
EK0858 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Systemic delivery of TNF-armed myxoma virus plus immune checkpoint inhibitor eliminates lung metastatic mouse osteosarcoma
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Mouse TNFSF4/OX40L ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated OX40 Ligand antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the OX40 Ligand antigen?
H. Lee
Verified customer
Asked: 2020-06-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-06-22
Question
Q: how do I analyze ELISA data? I have obtained OX40 Ligand level in serum.
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-03-04
Question
I purchased your EK0858 Mouse TNFSF4/OX40L ELISA Kit for IHC/IHC-Fr. Could you possibly share with me some images? Cheers!
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The product EK0858 Mouse TNFSF4/OX40L ELISA Kit was being tested using frozen human inflammatory lymph nodes by immunofluorescence resulting positive subgroup cells. We do not have an image of this EK0858 antibody in IHC-Fr.
Boster Scientific Support
Answered: 2020-03-03
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of OX40 Ligand in Mouse serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
L. Varma
Verified customer
Asked: 2019-09-12
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the OX40 Ligand ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-09-12
Question
Q: is it okay to use citrate plasma as samples in Mouse OX40 Ligand Picokine® ELISA Kit (Catalog # EK0858)?
W. Williams
Verified customer
Asked: 2019-07-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of OX40 Ligand causing degradation of its protein structure. OX40 Ligand may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the OX40 Ligand ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-07-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-06-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-06-22
Question
Q: which procedure should I follow in order to thaw whole blood sample for OX40 Ligand ELISA after freezing?
S. Roberts
Verified customer
Asked: 2019-06-10
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test OX40 Ligand for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-06-10
Question
Q: Can OX40 Ligand ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-11-12
