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Product Info Summary
| SKU: | PA1890 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Krueppel-like factor 8 KLF8 Antibody Picoband®
SKU/Catalog Number
PA1890
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Krueppel-like factor 8 KLF8 Antibody catalog # PA1890. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Krueppel-like factor 8 KLF8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1890)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human KLF8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1890 is reactive to KLF8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
39314 MW
Background of KLF8
KLF8 (Krueppel-like factor 8) is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins, including KLF8, all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the KLF8 gene to the X chromosome (WI-18614). Suske et al. (2005) stated that the human KLF8 gene maps to chromosome Xp11.21, and the mouse Klf8 gene to chromosome XF3. van Vliet et al. (2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser (PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1890 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Cell, 293T Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-KLF8 antibody, PA1890, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: 293T Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For KLF8 (Source: Uniprot.org, NCBI)
Gene Name
KLF8
Full Name
Krueppel-like factor 8
Weight
39314 MW
Superfamily
Sp1 C2H2-type zinc-finger protein family
Alternative Names
Krueppel-like factor 8;Basic krueppel-like factor 3;Zinc finger protein 741;KLF8;BKLF3, ZNF741; KLF8 BKLF3, ZNF741 Kruppel like factor 8 Krueppel-like factor 8|basic krueppel-like factor 3|basic kruppel-like factor 3|zinc finger protein 741
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF8, check out the KLF8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Krueppel-like factor 8 KLF8 Antibody Picoband® (PA1890)
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No publications found for PA1890
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Krueppel-like factor 8 KLF8 Antibody Picoband®
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for uterus using anti-KLF8 antibody PA1890. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-24
Question
Is a blocking peptide available for product anti-KLF8 antibody (PA1890)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
We do provide the blocking peptide for product anti-KLF8 antibody (PA1890). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-06
Question
I was wanting to use your anti-KLF8 antibody for WB for human uterus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human uterus identification?
A. Williams
Verified customer
Asked: 2018-12-27
Answer
As indicated on the product datasheet, PA1890 anti-KLF8 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-27
Question
Does PA1890 anti-KLF8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Kulkarni
Verified customer
Asked: 2016-07-07
Answer
As indicated on the product datasheet, PA1890 anti-KLF8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-07-07
Question
We are currently using anti-KLF8 antibody PA1890 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
E. Evans
Verified customer
Asked: 2016-02-08
Answer
The anti-KLF8 antibody (PA1890) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-08
Question
Is this PA1890 anti-KLF8 antibody reactive to the isotypes of KLF8?
K. Jha
Verified customer
Asked: 2013-05-03
Answer
The immunogen of PA1890 anti-KLF8 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human KLF8(1-18aa MVDMDKLINNLEVQLNSE). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-05-03
