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Product Info Summary
| SKU: | PA2156 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Transcription factor E2F4 E2F4 Antibody Picoband®
SKU/Catalog Number
PA2156
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcription factor E2F4 E2F4 Antibody catalog # PA2156. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor E2F4 E2F4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2156)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human E2F4, different from the related mouse and rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2156 is reactive to E2F4 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
43960 MW
Background of E2F-4
Transcription factor E2F4 is a protein that in humans is encoded by the E2F4 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. This gene is mapped to 16q22.1. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2156 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Lung Tissue, SMMC Whole Cell
Validation Images & Assay Conditions
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Anti-E2F4 antibody, PA2156, Western blotting
All lanes: Anti E2F4 (PA2156) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 44KD
Observed bind size: 44KD
Protein Target Info & Infographic
Gene/Protein Information For E2F4 (Source: Uniprot.org, NCBI)
Gene Name
E2F4
Full Name
Transcription factor E2F4
Weight
43960 MW
Superfamily
E2F/DP family
Alternative Names
Transcription factor E2F4;E2F-4;E2F4; E2F4 E2F-4 E2F transcription factor 4 transcription factor E2F4|E2F transcription factor 4, p107/p130-binding|p107/p130-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on E2F4, check out the E2F4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for E2F4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor E2F4 E2F4 Antibody Picoband® (PA2156)
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No publications found for PA2156
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Customer Q&As
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7 Customer Q&As for Anti-Transcription factor E2F4 E2F4 Antibody Picoband®
Question
Is this PA2156 anti-E2F4 antibody reactive to the isotypes of E2F4?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The immunogen of PA2156 anti-E2F4 antibody is A synthetic peptide corresponding to a sequence in the middle region of human E2F4(228-243aa LPKPALAQSQEASRPN), different from the related mouse and rat sequences by four amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-17
Question
I have a question about product PA2156, anti-E2F4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2156 anti-E2F4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-E2F4 antibody PA2156. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-07
Question
Our lab want to know about to test anti-E2F4 antibody PA2156 on human cervix carcinoma for research purposes, then I may be interested in using anti-E2F4 antibody PA2156 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-13
Answer
The products we sell, including anti-E2F4 antibody PA2156, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-13
Question
Does PA2156 anti-E2F4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Patel
Verified customer
Asked: 2017-05-03
Answer
It shows on the product datasheet, PA2156 anti-E2F4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-05-03
Question
Does anti-E2F4 antibody PA2156 work on goat WB with colon carcinoma?
B. Dhar
Verified customer
Asked: 2017-02-16
Answer
Our lab technicians have not validated anti-E2F4 antibody PA2156 on goat. You can run a BLAST between goat and the immunogen sequence of anti-E2F4 antibody PA2156 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat colon carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-16
Question
Would anti-E2F4 antibody PA2156 work for WB with cervix carcinoma?
B. Thomas
Verified customer
Asked: 2016-02-08
Answer
According to the expression profile of cervix carcinoma, E2F4 is highly expressed in cervix carcinoma. So, it is likely that anti-E2F4 antibody PA2156 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2016-02-08
