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Product Info Summary
| SKU: | PA1928 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-B MyB/MYBL2 Antibody Picoband®
SKU/Catalog Number
PA1928
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-B MyB/MYBL2 Antibody catalog # PA1928. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B MyB/MYBL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1928)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human B MyB, identical to the related mouse sequence and different from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1928 is reactive to MYBL2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
78764 MW
Background of B-Myb
MYBL2 (V-MYB avian myeloblastosis viral oncogene homolog-like 2), also called MYB-RELATED GENE BMYB, is a protein that in humans is encoded by the MYBL2 gene. The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. Barletta et al. (1991) assigned the MYBL2 gene to chromosome Xq13. However, Noben-Trauth et al. (1996) demonstrated that this assignment was an error. Using mouse Mybl2 cDNA clones as probes, they assigned Mybl2 in an interspecific backcross panel to distal mouse chromosome 2. Using human cDNA probes in combination with fluorescence in situ hybridization analysis, they localized MYBL2 to chromosome 20q13.1, a region that is commonly deleted in myeloid disorders and shows high homology of synteny to mouse chromosome 2. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1928 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Positive Control
WB: human Raji whole cell
IHC: human tonsil tissue, mouse liver tissue, rat liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MYBL2 using anti-MYBL2 antibody (PA1928).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYBL2 antigen affinity purified polyclonal antibody (Catalog # PA1928) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYBL2 at approximately 100 kDa. The expected band size for MYBL2 is at 79 kDa.
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Figure 2. IHC analysis of MYBL2 using anti-MYBL2 antibody (PA1928).
MYBL2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYBL2 Antibody (PA1928) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of MYBL2 using anti-MYBL2 antibody (PA1928).
MYBL2 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYBL2 Antibody (PA1928) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of MYBL2 using anti-MYBL2 antibody (PA1928).
MYBL2 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYBL2 Antibody (PA1928) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MYBL2 (Source: Uniprot.org, NCBI)
Gene Name
MYBL2
Full Name
Myb-related protein B
Weight
78764 MW
Alternative Names
Myb-related protein B;B-Myb;Myb-like protein 2;MYBL2;BMYB; MYBL2 B-MYB, BMYB MYB proto-oncogene like 2 myb-related protein B|myb-like protein 2|v-myb avian myeloblastosis viral oncogene homolog-like 2|v-myb myeloblastosis viral oncogene homolog-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYBL2, check out the MYBL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYBL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B MyB/MYBL2 Antibody Picoband® (PA1928)
Hello CJ!
PA1928 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Molecular mechanism of G1 arrest and cellular senescence induced by LEE011, a novel CDK4/CDK6 inhibitor, in leukemia cells
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Customer Q&As
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4 Customer Q&As for Anti-B MyB/MYBL2 Antibody Picoband®
Question
Will PA1928 anti-B MyB/MYBL2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
As indicated on the product datasheet, PA1928 anti-B MyB/MYBL2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-09
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-B MyB/MYBL2 antibody PA1928. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-23
Question
We are currently using anti-B MyB/MYBL2 antibody PA1928 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
The anti-B MyB/MYBL2 antibody (PA1928) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-19
Question
I am looking for to test anti-B MyB/MYBL2 antibody PA1928 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-B MyB/MYBL2 antibody PA1928 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Wu
Verified customer
Asked: 2017-10-17
Answer
The products we sell, including anti-B MyB/MYBL2 antibody PA1928, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-17
