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Product Info Summary
| SKU: | A00120 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-KLF4 Antibody Picoband®
SKU/Catalog Number
A00120
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KLF4 Antibody Picoband® catalog # A00120. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KLF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00120)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human KLF4, which shares 91.7% amino acid (aa) sequence identity with both mouse and rat KLF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00120 is reactive to KLF4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
54.671kDa
Background of KLF4
Kruppel-like factor 4, also known as EZF or GKLF, is a protein that in humans is encoded by the KLF4 gene. This gene is mapped to 9q31.2. KLF4 gene encodes a member of the Kruppel family of transcription factors. This gene plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. It is required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. This gene involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00120 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Positive Control
WB: human Hela whole cell, human COLO-320 whole cell, rat stomach tissue, rat testis tissue
IHC: rat small intestine tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KLF4 using anti-KLF4 antibody (A00120).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: rat testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF4 antigen affinity purified polyclonal antibody (Catalog # A00120) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF4 at approximately 60KD. The expected band size for KLF4 is at 55KD.
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Figure 2. IHC analysis of KLF4 using anti-KLF4 antibody (A00120).
KLF4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KLF4 Antibody (A00120) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of KLF4 using anti-KLF4 antibody (A00120).
KLF4 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-KLF4 Antibody (A00120) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For KLF4 (Source: Uniprot.org, NCBI)
Gene Name
KLF4
Full Name
Krueppel-like factor 4
Weight
54.671kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor; KLF4; EZF; GKLF KLF4 EZF, GKLF Kruppel like factor 4 Krueppel-like factor 4|Kruppel-like factor 4 (gut)|endothelial Kruppel-like zinc finger protein|epithelial zinc finger protein EZF|gut Kruppel-like factor|gut-enriched krueppel-like factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLF4, check out the KLF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KLF4 Antibody Picoband® (A00120)
Hello CJ!
A00120 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Deng S,Zhang Y,Wang Y,Lu X,Jiang Q.MicroRNA-92 regulates vascular smooth muscle cell function by targeting KLF4 during vascular restenosis and injury.Int J Clin Exp Pathol.2019 Dec 1;12(12):4253-4262.PMID:31933825;PMCID:PMC6949870.
Species: Human,Mouse
A00120 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION: 1:1000
Shi R,Liu L,Wang F,He Y,Niu Y,Wang C,Zhang X,Zhang X,Zhang H,Chen M,Wang Y.Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.Cell Signal.2020 Dec;76:109810.doi:10.1016/j.cellsig
Species: Human
A00120 usage in article: APP:WB, SAMPLE:MCF-7 CELL AND MDA-MB-231 CELL, DILUTION:NA
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901
CHIR99021 enhances Klf4 Expression through ?-Catenin Signaling and miR-7a Regulation in J1 Mouse Embryonic Stem Cells
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-KLF4 Antibody Picoband®
Question
We have observed staining in human colonic mucosa. What should we do? Is anti-KLF4 antibody supposed to stain colonic mucosa positively?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
From what I have seen in literature colonic mucosa does express KLF4. From what I have seen in Uniprot.org, KLF4 is expressed in colonic mucosa, placenta, substantia nigra tongue, cervix lung, cervix carcinoma, liver, among other tissues. Regarding which tissues have KLF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 18655026
Substantia nigra, and Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-28
Question
We ordered your anti-KLF4 antibody for IHC on liver in the past. I am using mouse, and I plan to use the antibody for WB next. We are interested in examining liver as well as substantia nigra tongue in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
I looked at the website and datasheets of our anti-KLF4 antibody and I see that A00120 has been tested on mouse in both IHC and WB. Thus A00120 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-31
Question
My boss were content with the WB result of your anti-KLF4 antibody. However we have been able to see positive staining in liver nucleus. using this antibody. Is that expected? Could you tell me where is KLF4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
According to literature, liver does express KLF4. Generally KLF4 expresses in nucleus. Regarding which tissues have KLF4 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Cervix, and Lung, Pubmed ID: 15489334
Liver, Pubmed ID: 18655026
Substantia nigra, and Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-12-13
Question
We are currently using anti-KLF4 antibody A00120 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
J. Rodriguez
Verified customer
Asked: 2019-10-09
Answer
The anti-KLF4 antibody (A00120) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-09
Question
I am interested in using your anti-KLF4 antibody for canonical wnt signaling pathway studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.
C. Moore
Verified customer
Asked: 2014-05-12
Answer
We appreciate your inquiry. This A00120 anti-KLF4 antibody is validated on human hela, hela whole cell lysates, rat stomach tissue, testis tissue, small intestine tissue, mouse spleen tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-05-12
