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Product Info Summary
| SKU: | A02187-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-KIR3DL1 Antibody Picoband®
SKU/Catalog Number
A02187-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KIR3DL1 Antibody Picoband® catalog # A02187-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-KIR3DL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02187-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KIR3DL1 recombinant protein (Position: Q26-N318).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02187-2 is reactive to KIR3DL1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
31568 MW
Background of KIR3DL1
Killer cell immunoglobulin-like receptor 3DL1 is a protein that in humans is encoded by the KIR3DL1 gene. Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02187-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of KIR3DL1 using anti-KIR3DL1 antibody (A02187-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIR3DL1 antigen affinity purified polyclonal antibody (Catalog # A02187-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIR3DL1 at approximately 49 kDa. The expected band size for KIR3DL1 is at 49 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KIR3DL1 (Source: Uniprot.org, NCBI)
Gene Name
KIR3DL1
Full Name
Killer cell immunoglobulin-like receptor 3DL1
Weight
31568 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Glycophorin-A; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD235a; GYPA; GPA KIR3DL1 CD158E1, KIR, KIR2DL5B/S1, NKAT-3, NKAT3, NKB1, NKB1B, KIR3DL1 killer cell immunoglobulin like receptor, three Ig domains and long cytoplasmic tail 1 killer cell immunoglobulin-like receptor 3DL1|CD158 -like family member E|HLA-BW4-specific inhibitory NK cell receptor|KIR 3DL1|killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1|natural killer-associated transcript 3|p70 NK receptor CL-2/CL-11|p70 killer cell inhibitory receptor|p70 natural killer cell receptor clones CL-2/CL-11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIR3DL1, check out the KIR3DL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIR3DL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KIR3DL1 Antibody Picoband® (A02187-2)
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