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Product Info Summary
| SKU: | A00254 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Ki67/Mki67 Antibody
View all Ki67/MKI67 Antibodies
SKU/Catalog Number
A00254
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ki67/Mki67 Antibody catalog # A00254. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ki67/Mki67 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00254)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Mki67 recombinant protein (Position: F29-S3177).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00254 is reactive to Mki67 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
358 kDa
Calculated molecular weight
358.694kDa
Background of Ki67/MKI67
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00254 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
IHC: human tonsil tissue, human lung cancer tissue, human thyroid cancer tissue, mouse thymus tissue, rat thymus tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (A00254).
Ki67/Mki67 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (A00254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Mki67 (Source: Uniprot.org, NCBI)
Gene Name
Mki67
Full Name
Proliferation marker protein Ki-67
Weight
358.694kDa
Alternative Names
Angiotensin-converting enzyme; ACE; Dipeptidyl carboxypeptidase I; Kininase II; CD143; Angiotensin-converting enzyme, soluble form; Ace; Dcp1 MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67| Ki67| by Ki-67|proliferation-related Ki-67 |protein phosphatase 1, regulatory subunit 105
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mki67, check out the Mki67 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mki67: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ki67/Mki67 Antibody (A00254)
Hello CJ!
A00254 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identification and differentiation therapy strategy of pterygium in vitro
Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium
Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing
Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter
Targeting MUS81 promotes the anticancer effect of WEE1 inhibitor and immune checkpoint blocking combination therapy via activating cGAS/STING signaling in gastric cancer cells
Leukemia inhibitory factor promotes tumor growth and metastasis in human osteosarcoma via activating STAT3
Low-level expression of let-7a in gastric cancer and its involvement in tumorigenesis by targeting RAB40C
Core-shell nanocarriers with high paclitaxel loading for passive and active targeting
Matrine promotes oligodendrocyte development in CNS autoimmunity through the PI3K/Akt signaling pathway
Quercetin inhibits angiogenesis by targeting calcineurin in the xenograft model of human breast cancer
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