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Product Info Summary
| SKU: | M00514 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-KEAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4]
SKU/Catalog Number
M00514
Size
100 µl
Description
Boster Bio KEAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4). Catalog# M00514. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-KEAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00514)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1B4
Isotype
IgG1
Immunogen
Full length human recombinnt protein of human KEAP1 (NP_987096) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00514 is reactive to KEAP1 in Human, Mouse, Rat
Applications
M00514 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).
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Figure from citation: Western Blot of Keap1 protein level by using anti-Keap1 antibody in human BEAS2B cells.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-KEAP1 monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-KEAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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HEK293T cells transfected with either KEAP1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody (M00514)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY KEAP1 using anti-KEAP1 antibody (M00514/green
Protein Target Info & Infographic
Gene/Protein Information For KEAP1 (Source: Uniprot.org, NCBI)
Gene Name
KEAP1
Full Name
Kelch-like ECH-associated protein 1
Weight
Superfamily
KEAP1 family
Alternative Names
INrf2; INrf2MGC9454; Keap1; kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132MGC4407; KLHL19Cytosolic inhibitor of Nrf2; MGC10630; MGC1114; MGC20887 KEAP1 INrf2, KLHL19 kelch like ECH associated protein 1 kelch-like ECH-associated protein 1|KEAP1 delta C|cytosolic inhibitor of Nrf2|kelch-like family member 19|kelch-like protein 19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KEAP1, check out the KEAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KEAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KEAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4] (M00514)
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