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Product Info Summary
| SKU: | A01723 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PAK4 Antibody
SKU/Catalog Number
A01723
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PAK4 Antibody (Catalog # A01723). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
PAK4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PAK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01723)
Host
Rabbit
Contents
PAK4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PAK4 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4. The immunogen is located within amino acids 210 - 260 of PAK4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PAK4 antibody is predicted to not cross-react with other PAK family proteins.
Reactive Species
A01723 is reactive to PAK4 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
64072 MW
Background of PAK4
The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01723 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PAK4 antibody can be used for the detection of PAK4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of PAK4 in human colon tissue with PAK4 antibody at 10 μg/mL.
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Immunofluorescence of PAK4 in Human Colon tissue with PAK4 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PAK4 (Source: Uniprot.org, NCBI)
Gene Name
PAK4
Full Name
Serine/threonine-protein kinase PAK 4
Weight
64072 MW
Superfamily
protein kinase superfamily
Alternative Names
KIAA1142, p21-activated kinase 4, PAK-4 PAK4 p21 (RAC1) activated kinase 4 serine/threonine-protein kinase PAK 4|p21 protein (Cdc42/Rac)-activated kinase 4|p21(CDKN1A)-activated kinase 4|protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PAK4, check out the PAK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PAK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PAK4 Antibody (A01723)
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